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- W2911699220 abstract "The product quality of recombinant proteins is of major importance for their intended purpose. The initial characterization of both simple and complex products should be performed as soon as practical. However, to comply with this high standard, appropriate selection of complementary methods is required. Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition. Methods applied for the determination of posttranslational modifications such as glycan analysis are not described. In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted." @default.
- W2911699220 created "2019-02-21" @default.
- W2911699220 creator A5058135636 @default.
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- W2911699220 date "2019-01-01" @default.
- W2911699220 modified "2023-09-23" @default.
- W2911699220 title "Assessing the Quality of Recombinant Products Made in Yeast" @default.
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- W2911699220 doi "https://doi.org/10.1007/978-1-4939-9024-5_19" @default.
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