FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2911759009> ?p ?o ?g. }

• W2911759009 abstract "Objectives: The mobile colistin resistance gene mcr-1 is a serious threat to global human and animal health. The composite transposon Tn6330 and its circular intermediate were proposed to be involved in the spread of mcr-1 but their roles remain poorly understood. Methods: To further explore the intermediates during the transposition of Tn6330, we engineered Escherichia coli strains that carry an intact Tn6330 transposon or its deletion derivatives. PCR assays were performed to detect IR-IR junctions and possible circular intermediates. We carried out transposition experiments to calculate transposition frequency. The transposition sites were characterized by whole genome sequence and ISMapper-based analyses. Results: The presence of an intact Tn6330 was demonstrated to be essential for the successful transposition of mcr-1, although both Tn6330 and Tn6330-ΔIR could form circular intermediates. The insertion sequence junction structure was observed in all constructed plasmids but the ISApl1 dimer was only formed in one construct containing an intact Tn6330. The average frequency of mcr-1 transposition in an E. coli strain possessing an intact Tn6330 was ∼10-6 per transformed cell. We identified 27 integration sites for the Tn6330 transposition event. All the transposition sites were flanked by 2 bp target duplications and preferentially occurred in AT-rich regions. Conclusion: These results indicate that mcr-1 transposition relies on the presence of an intact Tn6330. In addition, formation of the tandem repeat ISApl12 could represent a crucial intermediate. Taken together, the current investigations provide mechanistic insights in the transposition of mcr-1." @default.
• W2911759009 created "2019-02-21" @default.
• W2911759009 creator A5005106733 @default.
• W2911759009 creator A5028251869 @default.
• W2911759009 creator A5030285790 @default.
• W2911759009 creator A5031044364 @default.
• W2911759009 creator A5039384080 @default.
• W2911759009 creator A5048660993 @default.
• W2911759009 creator A5051097578 @default.
• W2911759009 creator A5052774470 @default.
• W2911759009 creator A5075518169 @default.
• W2911759009 creator A5077530505 @default.
• W2911759009 creator A5082435542 @default.
• W2911759009 date "2019-01-22" @default.
• W2911759009 modified "2023-09-26" @default.
• W2911759009 title "The ISApl12 Dimer Circular Intermediate Participates in mcr-1 Transposition" @default.
• W2911759009 cites W1997138603 @default.
• W2911759009 cites W2005024992 @default.
• W2911759009 cites W2064968987 @default.
• W2911759009 cites W2113068511 @default.
• W2911759009 cites W2116137883 @default.
• W2911759009 cites W2129933858 @default.
• W2911759009 cites W2146538795 @default.
• W2911759009 cites W216106564 @default.
• W2911759009 cites W2164032940 @default.
• W2911759009 cites W2179036997 @default.
• W2911759009 cites W2518717259 @default.
• W2911759009 cites W2524156053 @default.
• W2911759009 cites W2525708847 @default.
• W2911759009 cites W2559255286 @default.
• W2911759009 cites W2593444269 @default.
• W2911759009 cites W2603405734 @default.
• W2911759009 cites W2606564392 @default.
• W2911759009 cites W2743038938 @default.
• W2911759009 cites W2765222305 @default.
• W2911759009 cites W2770937934 @default.
• W2911759009 cites W2772875632 @default.
• W2911759009 cites W2786916519 @default.
• W2911759009 cites W2789500779 @default.
• W2911759009 cites W2789944246 @default.
• W2911759009 cites W2796584644 @default.
• W2911759009 cites W2802440124 @default.
• W2911759009 cites W2804179409 @default.
• W2911759009 cites W2884501756 @default.
• W2911759009 cites W866184829 @default.
• W2911759009 doi "https://doi.org/10.3389/fmicb.2019.00015" @default.
• W2911759009 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6349718" @default.
• W2911759009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30723461" @default.
• W2911759009 hasPublicationYear "2019" @default.
• W2911759009 type Work @default.
• W2911759009 sameAs 2911759009 @default.
• W2911759009 citedByCount "24" @default.
• W2911759009 countsByYear W29117590092019 @default.
• W2911759009 countsByYear W29117590092020 @default.
• W2911759009 countsByYear W29117590092021 @default.
• W2911759009 countsByYear W29117590092022 @default.
• W2911759009 countsByYear W29117590092023 @default.
• W2911759009 crossrefType "journal-article" @default.
• W2911759009 hasAuthorship W2911759009A5005106733 @default.
• W2911759009 hasAuthorship W2911759009A5028251869 @default.
• W2911759009 hasAuthorship W2911759009A5030285790 @default.
• W2911759009 hasAuthorship W2911759009A5031044364 @default.
• W2911759009 hasAuthorship W2911759009A5039384080 @default.
• W2911759009 hasAuthorship W2911759009A5048660993 @default.
• W2911759009 hasAuthorship W2911759009A5051097578 @default.
• W2911759009 hasAuthorship W2911759009A5052774470 @default.
• W2911759009 hasAuthorship W2911759009A5075518169 @default.
• W2911759009 hasAuthorship W2911759009A5077530505 @default.
• W2911759009 hasAuthorship W2911759009A5082435542 @default.
• W2911759009 hasBestOaLocation W29117590091 @default.
• W2911759009 hasConcept C104317684 @default.
• W2911759009 hasConcept C12455157 @default.
• W2911759009 hasConcept C138885662 @default.
• W2911759009 hasConcept C141231307 @default.
• W2911759009 hasConcept C157887575 @default.
• W2911759009 hasConcept C172510086 @default.
• W2911759009 hasConcept C22744801 @default.
• W2911759009 hasConcept C41895202 @default.
• W2911759009 hasConcept C4918238 @default.
• W2911759009 hasConcept C51603236 @default.
• W2911759009 hasConcept C54355233 @default.
• W2911759009 hasConcept C86803240 @default.
• W2911759009 hasConceptScore W2911759009C104317684 @default.
• W2911759009 hasConceptScore W2911759009C12455157 @default.
• W2911759009 hasConceptScore W2911759009C138885662 @default.
• W2911759009 hasConceptScore W2911759009C141231307 @default.
• W2911759009 hasConceptScore W2911759009C157887575 @default.
• W2911759009 hasConceptScore W2911759009C172510086 @default.
• W2911759009 hasConceptScore W2911759009C22744801 @default.
• W2911759009 hasConceptScore W2911759009C41895202 @default.
• W2911759009 hasConceptScore W2911759009C4918238 @default.
• W2911759009 hasConceptScore W2911759009C51603236 @default.
• W2911759009 hasConceptScore W2911759009C54355233 @default.
• W2911759009 hasConceptScore W2911759009C86803240 @default.
• W2911759009 hasLocation W29117590091 @default.
• W2911759009 hasLocation W29117590092 @default.
• W2911759009 hasLocation W29117590093 @default.
• W2911759009 hasLocation W29117590094 @default.
• W2911759009 hasOpenAccess W2911759009 @default.
• W2911759009 hasPrimaryLocation W29117590091 @default.

• next