Matches in SemOpenAlex for { <https://semopenalex.org/work/W2911797894> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2911797894 endingPage "52a" @default.
- W2911797894 startingPage "52a" @default.
- W2911797894 abstract "The serotonin transporter (SERT), a monoamine transporter family member, controls the magnitude and duration of serotonergic neurotransmission by facilitating reuptake of serotonin back into the pre-synaptic neuron. Selective serotonin re-uptake inhibitors that prolong neurotransmitter signaling have been developed to treat depression and other conditions. However, these drugs are not always effective and more specific and efficacious therapeutics could result from a better understanding of the structure and mechanistic function of SERT. The structures of SERT and related transporters have been determined and serve as useful structural models; however they are truncated for thermostability and typically lack significant regions of the extracellular loops and the amino- and carboxy-termini. This study directly interrogates the topology of the extracellular loops of full-length SERT in its resting state utilizing site-directed photoaffinity labeling and mass spectrometry (MS) to provide distance constraints. In every extracellular loop at least one single cysteine mutation was introduced systematically, using a Cys-null rat SERT construct and overexpression in Sf-9 insect cells. For each mutation, purified rSERT was reconstituted into lipid vesicles and tagged with MTS-benzophenone. Upon photoactivation isolated rSERT was proteolyzed and analyzed by tandem MS studies to identify sites of non-specific covalent attachment of the photocrosslinker. The network of identified crosslinks provides direct distance constraints and direct topological information regarding loop structure, offering a unique complement to high resolution structural determinations. ." @default.
- W2911797894 created "2019-02-21" @default.
- W2911797894 creator A5028452792 @default.
- W2911797894 date "2019-02-01" @default.
- W2911797894 modified "2023-09-28" @default.
- W2911797894 title "Mapping the Extracellular Loops of the Serotonin Transporter Using Crosslinking-Mass Spectrometry" @default.
- W2911797894 doi "https://doi.org/10.1016/j.bpj.2018.11.327" @default.
- W2911797894 hasPublicationYear "2019" @default.
- W2911797894 type Work @default.
- W2911797894 sameAs 2911797894 @default.
- W2911797894 citedByCount "0" @default.
- W2911797894 crossrefType "journal-article" @default.
- W2911797894 hasAuthorship W2911797894A5028452792 @default.
- W2911797894 hasBestOaLocation W29117978941 @default.
- W2911797894 hasConcept C104317684 @default.
- W2911797894 hasConcept C12554922 @default.
- W2911797894 hasConcept C149011108 @default.
- W2911797894 hasConcept C16696662 @default.
- W2911797894 hasConcept C170493617 @default.
- W2911797894 hasConcept C185592680 @default.
- W2911797894 hasConcept C187714386 @default.
- W2911797894 hasConcept C200170125 @default.
- W2911797894 hasConcept C2775864247 @default.
- W2911797894 hasConcept C2776219046 @default.
- W2911797894 hasConcept C2778897192 @default.
- W2911797894 hasConcept C28406088 @default.
- W2911797894 hasConcept C2908747647 @default.
- W2911797894 hasConcept C2993285035 @default.
- W2911797894 hasConcept C37000724 @default.
- W2911797894 hasConcept C55493867 @default.
- W2911797894 hasConcept C86803240 @default.
- W2911797894 hasConcept C95444343 @default.
- W2911797894 hasConcept C99631742 @default.
- W2911797894 hasConceptScore W2911797894C104317684 @default.
- W2911797894 hasConceptScore W2911797894C12554922 @default.
- W2911797894 hasConceptScore W2911797894C149011108 @default.
- W2911797894 hasConceptScore W2911797894C16696662 @default.
- W2911797894 hasConceptScore W2911797894C170493617 @default.
- W2911797894 hasConceptScore W2911797894C185592680 @default.
- W2911797894 hasConceptScore W2911797894C187714386 @default.
- W2911797894 hasConceptScore W2911797894C200170125 @default.
- W2911797894 hasConceptScore W2911797894C2775864247 @default.
- W2911797894 hasConceptScore W2911797894C2776219046 @default.
- W2911797894 hasConceptScore W2911797894C2778897192 @default.
- W2911797894 hasConceptScore W2911797894C28406088 @default.
- W2911797894 hasConceptScore W2911797894C2908747647 @default.
- W2911797894 hasConceptScore W2911797894C2993285035 @default.
- W2911797894 hasConceptScore W2911797894C37000724 @default.
- W2911797894 hasConceptScore W2911797894C55493867 @default.
- W2911797894 hasConceptScore W2911797894C86803240 @default.
- W2911797894 hasConceptScore W2911797894C95444343 @default.
- W2911797894 hasConceptScore W2911797894C99631742 @default.
- W2911797894 hasIssue "3" @default.
- W2911797894 hasLocation W29117978941 @default.
- W2911797894 hasOpenAccess W2911797894 @default.
- W2911797894 hasPrimaryLocation W29117978941 @default.
- W2911797894 hasRelatedWork W1579298346 @default.
- W2911797894 hasRelatedWork W1606818667 @default.
- W2911797894 hasRelatedWork W1973529237 @default.
- W2911797894 hasRelatedWork W1998148889 @default.
- W2911797894 hasRelatedWork W2209727521 @default.
- W2911797894 hasRelatedWork W2352859200 @default.
- W2911797894 hasRelatedWork W2431978283 @default.
- W2911797894 hasRelatedWork W2490526596 @default.
- W2911797894 hasRelatedWork W3016172228 @default.
- W2911797894 hasRelatedWork W2131146428 @default.
- W2911797894 hasVolume "116" @default.
- W2911797894 isParatext "false" @default.
- W2911797894 isRetracted "false" @default.
- W2911797894 magId "2911797894" @default.
- W2911797894 workType "article" @default.