Matches in SemOpenAlex for { <https://semopenalex.org/work/W2911951162> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2911951162 endingPage "263a" @default.
- W2911951162 startingPage "262a" @default.
- W2911951162 abstract "Missense mutation Ile79Asn in human cardiac troponin T (HcTnT-I79N) has been linked to phenotypic outcomes of hypertrophic cardiomyopathy (HCM) followed by adverse consequences including arrhythmias and sudden cardiac death. Little else is known about changes in cardiac muscle mechanics caused by the mutation. This study investigates cross-bridges kinetics of left ventricular papillary muscle bundles (Ca2+-sensitivity of isometric force, sinusoidal stiffness and rate of tension redevelopment (kTR)). Cardiac tissues were harvested from both non-transgenic wild-type (NTg) mice and transgenic mice bearing the HcTnT-I79N mutation. Sarcomere lengths (1.9, 2.1, and 2.3 μm) were set at pCa 8.0 using HeNe laser. Sinusoidal stiffness (0.2% PTP length oscillation) and kTR were obtained during Ca2+ activation when force was at least 20% of maximum. Increased Ca2+-sensitivity of isometric force was observed in NTg cardiac preparations upon an increase in SL (1.9 vs 2.1 μm, and 2.1 vs 2.3 μm). Interestingly, HcTnT-I79N exhibited an increased Ca2+-sensitivity at SL 1.9 vs 2.1 μm only. No further increase was observed at SL 2.1 vs 2.3 μm. This blunt effect indicates an abolishment of length-dependent activation at SL 2.1 vs 2.3 μm. Although HcTnT-I79N mutation exhibited a significant lower maximal kTR, neither NTg nor HcTnT-I79N showed significant changes in maximal kTR regarding length-dependent activation. After compressing the myofilament lattice spacing using 3% Dextran T500 (SL 2.1 μm), only NTg cardiac preparations displayed increased myofilament Ca2+ sensitivity. No changes in maximal kTR were observed for both NTg and HcTnT-I79N in the presence of dextran. In addition, no significant changes in passive force, maximal force and cooperativity of thin filament activation were observed between groups. Experiments were carried out at ∼21°C. Analysis of mathematical modeling of force and sinusoidal stiffness are in progress. NIH-HL128683." @default.
- W2911951162 created "2019-02-21" @default.
- W2911951162 creator A5030863338 @default.
- W2911951162 creator A5032333520 @default.
- W2911951162 creator A5034740001 @default.
- W2911951162 creator A5035103183 @default.
- W2911951162 creator A5064477997 @default.
- W2911951162 date "2019-02-01" @default.
- W2911951162 modified "2023-10-02" @default.
- W2911951162 title "Annulment of Cardiac Muscle Length-Dependent Force Activation in Transgenic Mice Bearing the HcTnT-I79N Mutation" @default.
- W2911951162 doi "https://doi.org/10.1016/j.bpj.2018.11.1427" @default.
- W2911951162 hasPublicationYear "2019" @default.
- W2911951162 type Work @default.
- W2911951162 sameAs 2911951162 @default.
- W2911951162 citedByCount "0" @default.
- W2911951162 crossrefType "journal-article" @default.
- W2911951162 hasAuthorship W2911951162A5030863338 @default.
- W2911951162 hasAuthorship W2911951162A5032333520 @default.
- W2911951162 hasAuthorship W2911951162A5034740001 @default.
- W2911951162 hasAuthorship W2911951162A5035103183 @default.
- W2911951162 hasAuthorship W2911951162A5064477997 @default.
- W2911951162 hasBestOaLocation W29119511621 @default.
- W2911951162 hasConcept C103486182 @default.
- W2911951162 hasConcept C104317684 @default.
- W2911951162 hasConcept C12554922 @default.
- W2911951162 hasConcept C126322002 @default.
- W2911951162 hasConcept C134018914 @default.
- W2911951162 hasConcept C164705383 @default.
- W2911951162 hasConcept C174054327 @default.
- W2911951162 hasConcept C185592680 @default.
- W2911951162 hasConcept C207200792 @default.
- W2911951162 hasConcept C2777274916 @default.
- W2911951162 hasConcept C2778996579 @default.
- W2911951162 hasConcept C2780185194 @default.
- W2911951162 hasConcept C36036425 @default.
- W2911951162 hasConcept C500558357 @default.
- W2911951162 hasConcept C501734568 @default.
- W2911951162 hasConcept C55493867 @default.
- W2911951162 hasConcept C68731436 @default.
- W2911951162 hasConcept C71924100 @default.
- W2911951162 hasConcept C75563809 @default.
- W2911951162 hasConcept C86803240 @default.
- W2911951162 hasConceptScore W2911951162C103486182 @default.
- W2911951162 hasConceptScore W2911951162C104317684 @default.
- W2911951162 hasConceptScore W2911951162C12554922 @default.
- W2911951162 hasConceptScore W2911951162C126322002 @default.
- W2911951162 hasConceptScore W2911951162C134018914 @default.
- W2911951162 hasConceptScore W2911951162C164705383 @default.
- W2911951162 hasConceptScore W2911951162C174054327 @default.
- W2911951162 hasConceptScore W2911951162C185592680 @default.
- W2911951162 hasConceptScore W2911951162C207200792 @default.
- W2911951162 hasConceptScore W2911951162C2777274916 @default.
- W2911951162 hasConceptScore W2911951162C2778996579 @default.
- W2911951162 hasConceptScore W2911951162C2780185194 @default.
- W2911951162 hasConceptScore W2911951162C36036425 @default.
- W2911951162 hasConceptScore W2911951162C500558357 @default.
- W2911951162 hasConceptScore W2911951162C501734568 @default.
- W2911951162 hasConceptScore W2911951162C55493867 @default.
- W2911951162 hasConceptScore W2911951162C68731436 @default.
- W2911951162 hasConceptScore W2911951162C71924100 @default.
- W2911951162 hasConceptScore W2911951162C75563809 @default.
- W2911951162 hasConceptScore W2911951162C86803240 @default.
- W2911951162 hasIssue "3" @default.
- W2911951162 hasLocation W29119511621 @default.
- W2911951162 hasOpenAccess W2911951162 @default.
- W2911951162 hasPrimaryLocation W29119511621 @default.
- W2911951162 hasRelatedWork W1976242910 @default.
- W2911951162 hasRelatedWork W2001959278 @default.
- W2911951162 hasRelatedWork W2014664809 @default.
- W2911951162 hasRelatedWork W2017636481 @default.
- W2911951162 hasRelatedWork W2069855479 @default.
- W2911951162 hasRelatedWork W2170842924 @default.
- W2911951162 hasRelatedWork W2290766369 @default.
- W2911951162 hasRelatedWork W3215121734 @default.
- W2911951162 hasRelatedWork W4256524488 @default.
- W2911951162 hasRelatedWork W4319936813 @default.
- W2911951162 hasVolume "116" @default.
- W2911951162 isParatext "false" @default.
- W2911951162 isRetracted "false" @default.
- W2911951162 magId "2911951162" @default.
- W2911951162 workType "article" @default.