Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912004382> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2912004382 abstract "Peri-infarct spreading depolarization (PIDs) frequently occur post ischemic stroke in animals and humans, propagating from the infarct core into the penumbra, and have been associated with worsened neurological outcome in patients. The etiology of PIDs is not well understood, complicating the development of targeted treatments. To examine the cellular origins of PIDs, we hypothesized that synchronous, frequency-specific, activation of specific neuronal subpopulation is necessary for PID occurrence. We transfected adult male Sprague Dawley rats with ChR2 either under the CamKII (AAV5.CAMKII.ArchT.GFP.WPRE.SV40) or CAG (AAV5.CAG.hChR2(H134R)-mCherry.WPRE.SV40) promoters into the somatosensory cortex to induce opsin transfection in pyramidal neurons or interneurons, respectively. Two weeks later, we assessed neuronal activity changes induced by focal cortical ischemia by inserting two electrodes ~2 mm apart in rostro-caudal direction. We then used a glass micropipette to inject 800 pmol of ET-1 into the primary somatosensory cortex in correspondence of rostral electrode, so as to examine the activity at the ‘core’ of the ischemic insult as well as from the peri-infarct zone, which coincided with the transfected area. We recorded baseline (pre-ischemic) local field potentials (LFPs) before injecting ET-1. Following ET-1 injection, we observed an average of five CSDs, with a mean amplitude of 57 mV and a mean duration of 1.2min, propagating from the infarct core to the peri-infarct tissue. In all subjects, the CSDs subsided within the first hour after the ischemic insult. In the second hour following stroke, we stimulated peri-infarct pyramidal neurons or interneurons at different frequency bands (theta, alpha, beta, low gamma, high gamma, and DC). Only theta band stimulation of the pyramidal neurons reliably triggered PIDs. Stimulating pyramidal neurons at higher frequencies or interneurons at any frequency did not elicit PIDs. Activation of peri-infarct zone pyramidal neurons in the theta band elicits cortical PIDs. The identification of a neuronal-specific and frequency-specific trigger for PIDs provides a roadmap for development of PID-targeted treatments in the acute stage of stroke." @default.
- W2912004382 created "2019-02-21" @default.
- W2912004382 creator A5030086855 @default.
- W2912004382 creator A5041218352 @default.
- W2912004382 creator A5044113968 @default.
- W2912004382 creator A5052626998 @default.
- W2912004382 creator A5055634028 @default.
- W2912004382 creator A5068634652 @default.
- W2912004382 date "2019-02-01" @default.
- W2912004382 modified "2023-10-09" @default.
- W2912004382 title "Abstract 158: Optogenetic Theta-band Activation of Pyramidal Neurons Elicits Peri-infarct Spreading Depolarizations in the ET-1 Rat Model of Focal Cortical Ischemia" @default.
- W2912004382 doi "https://doi.org/10.1161/str.50.suppl_1.158" @default.
- W2912004382 hasPublicationYear "2019" @default.
- W2912004382 type Work @default.
- W2912004382 sameAs 2912004382 @default.
- W2912004382 citedByCount "0" @default.
- W2912004382 crossrefType "journal-article" @default.
- W2912004382 hasAuthorship W2912004382A5030086855 @default.
- W2912004382 hasAuthorship W2912004382A5041218352 @default.
- W2912004382 hasAuthorship W2912004382A5044113968 @default.
- W2912004382 hasAuthorship W2912004382A5052626998 @default.
- W2912004382 hasAuthorship W2912004382A5055634028 @default.
- W2912004382 hasAuthorship W2912004382A5068634652 @default.
- W2912004382 hasConcept C117838684 @default.
- W2912004382 hasConcept C118552586 @default.
- W2912004382 hasConcept C122356138 @default.
- W2912004382 hasConcept C126322002 @default.
- W2912004382 hasConcept C127413603 @default.
- W2912004382 hasConcept C169760540 @default.
- W2912004382 hasConcept C172497186 @default.
- W2912004382 hasConcept C2777222312 @default.
- W2912004382 hasConcept C2777348757 @default.
- W2912004382 hasConcept C2777798775 @default.
- W2912004382 hasConcept C2778541695 @default.
- W2912004382 hasConcept C2780577055 @default.
- W2912004382 hasConcept C2780645631 @default.
- W2912004382 hasConcept C4141045 @default.
- W2912004382 hasConcept C42219234 @default.
- W2912004382 hasConcept C500558357 @default.
- W2912004382 hasConcept C50738837 @default.
- W2912004382 hasConcept C541997718 @default.
- W2912004382 hasConcept C71924100 @default.
- W2912004382 hasConcept C78519656 @default.
- W2912004382 hasConcept C86803240 @default.
- W2912004382 hasConceptScore W2912004382C117838684 @default.
- W2912004382 hasConceptScore W2912004382C118552586 @default.
- W2912004382 hasConceptScore W2912004382C122356138 @default.
- W2912004382 hasConceptScore W2912004382C126322002 @default.
- W2912004382 hasConceptScore W2912004382C127413603 @default.
- W2912004382 hasConceptScore W2912004382C169760540 @default.
- W2912004382 hasConceptScore W2912004382C172497186 @default.
- W2912004382 hasConceptScore W2912004382C2777222312 @default.
- W2912004382 hasConceptScore W2912004382C2777348757 @default.
- W2912004382 hasConceptScore W2912004382C2777798775 @default.
- W2912004382 hasConceptScore W2912004382C2778541695 @default.
- W2912004382 hasConceptScore W2912004382C2780577055 @default.
- W2912004382 hasConceptScore W2912004382C2780645631 @default.
- W2912004382 hasConceptScore W2912004382C4141045 @default.
- W2912004382 hasConceptScore W2912004382C42219234 @default.
- W2912004382 hasConceptScore W2912004382C500558357 @default.
- W2912004382 hasConceptScore W2912004382C50738837 @default.
- W2912004382 hasConceptScore W2912004382C541997718 @default.
- W2912004382 hasConceptScore W2912004382C71924100 @default.
- W2912004382 hasConceptScore W2912004382C78519656 @default.
- W2912004382 hasConceptScore W2912004382C86803240 @default.
- W2912004382 hasIssue "Suppl_1" @default.
- W2912004382 hasLocation W29120043821 @default.
- W2912004382 hasOpenAccess W2912004382 @default.
- W2912004382 hasPrimaryLocation W29120043821 @default.
- W2912004382 hasRelatedWork W2010417538 @default.
- W2912004382 hasRelatedWork W2083223563 @default.
- W2912004382 hasRelatedWork W2166512953 @default.
- W2912004382 hasRelatedWork W2238892548 @default.
- W2912004382 hasRelatedWork W2520243202 @default.
- W2912004382 hasRelatedWork W2912004382 @default.
- W2912004382 hasRelatedWork W2969325811 @default.
- W2912004382 hasRelatedWork W3016186531 @default.
- W2912004382 hasRelatedWork W3182936681 @default.
- W2912004382 hasRelatedWork W4297813335 @default.
- W2912004382 hasVolume "50" @default.
- W2912004382 isParatext "false" @default.
- W2912004382 isRetracted "false" @default.
- W2912004382 magId "2912004382" @default.
- W2912004382 workType "article" @default.