Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912031968> ?p ?o ?g. }
- W2912031968 endingPage "1303" @default.
- W2912031968 startingPage "1292" @default.
- W2912031968 abstract "Abstract Widespread therapeutic and commercial interest in recombinant mucin technology has emerged due to the unique ability of mucin glycoproteins to hydrate, protect, and lubricate biological surfaces. However, recombinant production of the large, highly repetitive domains that are characteristic of mucins remains a challenge in biomanufacturing likely due, at least in part, to the inherent instability of DNA repeats in the cellular genome. To overcome this challenge, we exploit codon redundancy to encode desired mucin polypeptides with minimal nucleotide repetition. The codon‐scrambling strategy was applied to generate synonymous genes, or “synDNAs,” for two mucins of commercial interest: lubricin and mucin 1. Stable, long‐term recombinant production in suspension‐adapted human 293‐F cells was demonstrated for the synonymous lubricin complementary DNA (cDNA), which we refer to as SynLubricin. Under optimal conditions, a 293‐F subpopulation produced recombinant SynLubricin at more than 200 mg/L of media and was stable throughout 2 months of continuous culture. Functionality tests confirmed that the recombinant lubricin could effectively inhibit cell adhesion and lubricate cartilage explants. Together, our work provides a viable workflow for cDNA design and stable mucin production in mammalian host production systems." @default.
- W2912031968 created "2019-02-21" @default.
- W2912031968 creator A5007182406 @default.
- W2912031968 creator A5007546671 @default.
- W2912031968 creator A5011554115 @default.
- W2912031968 creator A5021365493 @default.
- W2912031968 creator A5023158092 @default.
- W2912031968 creator A5028139581 @default.
- W2912031968 creator A5052206494 @default.
- W2912031968 creator A5053906449 @default.
- W2912031968 creator A5071630308 @default.
- W2912031968 creator A5072103120 @default.
- W2912031968 creator A5087621922 @default.
- W2912031968 date "2019-03-08" @default.
- W2912031968 modified "2023-10-15" @default.
- W2912031968 title "Stable recombinant production of codon‐scrambled lubricin and mucin in human cells" @default.
- W2912031968 cites W1532082365 @default.
- W2912031968 cites W1604406067 @default.
- W2912031968 cites W1915519529 @default.
- W2912031968 cites W1963996129 @default.
- W2912031968 cites W1975304740 @default.
- W2912031968 cites W1976996315 @default.
- W2912031968 cites W1978739865 @default.
- W2912031968 cites W1992773045 @default.
- W2912031968 cites W1994354502 @default.
- W2912031968 cites W1997984454 @default.
- W2912031968 cites W2003885329 @default.
- W2912031968 cites W2010655741 @default.
- W2912031968 cites W2014415867 @default.
- W2912031968 cites W2015769931 @default.
- W2912031968 cites W2017355698 @default.
- W2912031968 cites W2029583307 @default.
- W2912031968 cites W2037606715 @default.
- W2912031968 cites W2073775017 @default.
- W2912031968 cites W2082768611 @default.
- W2912031968 cites W2089509133 @default.
- W2912031968 cites W2090816971 @default.
- W2912031968 cites W2093599867 @default.
- W2912031968 cites W2099467894 @default.
- W2912031968 cites W2105826664 @default.
- W2912031968 cites W2112814753 @default.
- W2912031968 cites W2117471612 @default.
- W2912031968 cites W2130314959 @default.
- W2912031968 cites W2131598060 @default.
- W2912031968 cites W2133599168 @default.
- W2912031968 cites W2144475884 @default.
- W2912031968 cites W2149459456 @default.
- W2912031968 cites W2150173080 @default.
- W2912031968 cites W2167279371 @default.
- W2912031968 cites W2258324010 @default.
- W2912031968 cites W2346750548 @default.
- W2912031968 cites W2415805387 @default.
- W2912031968 cites W2504094337 @default.
- W2912031968 cites W2549168513 @default.
- W2912031968 cites W2592883798 @default.
- W2912031968 cites W2776567026 @default.
- W2912031968 cites W2811346354 @default.
- W2912031968 cites W4254025084 @default.
- W2912031968 cites W4378744814 @default.
- W2912031968 doi "https://doi.org/10.1002/bit.26940" @default.
- W2912031968 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6764099" @default.
- W2912031968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30684357" @default.
- W2912031968 hasPublicationYear "2019" @default.
- W2912031968 type Work @default.
- W2912031968 sameAs 2912031968 @default.
- W2912031968 citedByCount "9" @default.
- W2912031968 countsByYear W29120319682019 @default.
- W2912031968 countsByYear W29120319682020 @default.
- W2912031968 countsByYear W29120319682021 @default.
- W2912031968 countsByYear W29120319682023 @default.
- W2912031968 crossrefType "journal-article" @default.
- W2912031968 hasAuthorship W2912031968A5007182406 @default.
- W2912031968 hasAuthorship W2912031968A5007546671 @default.
- W2912031968 hasAuthorship W2912031968A5011554115 @default.
- W2912031968 hasAuthorship W2912031968A5021365493 @default.
- W2912031968 hasAuthorship W2912031968A5023158092 @default.
- W2912031968 hasAuthorship W2912031968A5028139581 @default.
- W2912031968 hasAuthorship W2912031968A5052206494 @default.
- W2912031968 hasAuthorship W2912031968A5053906449 @default.
- W2912031968 hasAuthorship W2912031968A5071630308 @default.
- W2912031968 hasAuthorship W2912031968A5072103120 @default.
- W2912031968 hasAuthorship W2912031968A5087621922 @default.
- W2912031968 hasBestOaLocation W29120319682 @default.
- W2912031968 hasConcept C104317684 @default.
- W2912031968 hasConcept C108625454 @default.
- W2912031968 hasConcept C153911025 @default.
- W2912031968 hasConcept C179264091 @default.
- W2912031968 hasConcept C2777421810 @default.
- W2912031968 hasConcept C40767141 @default.
- W2912031968 hasConcept C55493867 @default.
- W2912031968 hasConcept C70721500 @default.
- W2912031968 hasConcept C86803240 @default.
- W2912031968 hasConcept C95444343 @default.
- W2912031968 hasConceptScore W2912031968C104317684 @default.
- W2912031968 hasConceptScore W2912031968C108625454 @default.
- W2912031968 hasConceptScore W2912031968C153911025 @default.
- W2912031968 hasConceptScore W2912031968C179264091 @default.
- W2912031968 hasConceptScore W2912031968C2777421810 @default.