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- W2912046715 abstract "Single molecule studies aim at extracting desirable measurements, such as locations and orientations, from single-molecule emission patterns. Numerous algorithms and microscopy techniques have been developed over the last decades towards this aim in the aspect of either increasing the measurement dimensions or the estimation precisions. However, without considering the detailed designs of a data analyzing algorithm or a microscope system, the amount of information contained in a given single-molecule emission pattern can be quantified using the Fisher information matrix which provides a lower bound of the measurement uncertainty, named Cramé-Rao lower bound (CRLB). Here we examined the CRLB of estimating various information carried by single-molecule emission patterns, such as molecule locations and orientations, and wavefront distortions, from several types of existing point spread functions, such as astigmatism PSF, double-helix PSF, dipole PSF, interferometric 4Pi PSF and PSFs that were distorted by systematic or sample induced aberrations. Furthermore, from the perspective of information theory, we also evaluated the change of information content by various image processing techniques such as noise reduction and super-resolution microscopy reconstruction." @default.
- W2912046715 created "2019-02-21" @default.
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- W2912046715 date "2019-02-01" @default.
- W2912046715 modified "2023-10-16" @default.
- W2912046715 title "Single Molecule Measurements Based on Information Theory" @default.
- W2912046715 doi "https://doi.org/10.1016/j.bpj.2018.11.755" @default.
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