FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912088003> ?p ?o ?g. }

• W2912088003 abstract "Hematopoietic stem cells (HSCs) are blood forming cells which possess the ability to differentiate into all types of blood cells. T cells are one important cell type HSCs can differentiate into, via corresponding progenitor cells. T cells are part of the adaptive immune system as they mediate cellular immune responses. Due to this crucial function, in vitro differentiated T cells are subject of current studies in the biomedical field in terms of cell transplantation. Studies show that the density of the immobilized Notch ligand Delta-like 1 (DLL1) presented in HSCs´ environment can stimulate their differentiation toward T cells. The development of reliable synthetic cell culture systems presenting variable densities of DLL1 is promising for the future expansion of T cells´ clinical applications. Here we introduce bifunctional polyethylene glycol-based (PEG-based) hydrogels as potential instructing platform for the differentiation of human hematopoietic stem and progenitor cells (HSPCs) to T cells. PEG hydrogels bearing the cell adhesion supporting motif RGD (arginyl-glycyl-aspartic acid) were synthesized by UV-light induced radical copolymerization of PEG diacrylate and RGD modified PEG acrylate. The hydrogels were furthermore nanostructured by incorporation of gold nanoparticle arrays that were produced by block copolymer micelle nanolithography (BCML). BCML allows for the decoration of surfaces with gold nanoparticles in a hexagonal manner with well-defined interparticle distances. To determine the impact of DLL1 density on the cell differentiation, hydrogels with particle distances of approximately 40 nm and 90 nm were synthesized and the gold nanoparticles were functionalized with DLL1. After 27 days in culture, HSPCs showed an unphysiological differentiation status and, therefore, the differentiation was evaluated as atypical T lymphoid differentiation. Cluster of differentiation (CD) 4 was the only tested T cell marker which was expressed clearly in all samples. Thus, although the applied nanopatterned hydrogels affected two important signaling pathways (integrins and Notch) for T cell differentiation, it appears that more functionalities that control T cell differentiation in nature need to be considered for achieving fully synthetic in vitro T cell differentiation strategies." @default.
• W2912088003 created "2019-02-21" @default.
• W2912088003 creator A5048451756 @default.
• W2912088003 creator A5059581407 @default.
• W2912088003 creator A5073452439 @default.
• W2912088003 creator A5077987843 @default.
• W2912088003 creator A5089921462 @default.
• W2912088003 date "2019-01-29" @default.
• W2912088003 modified "2023-09-30" @default.
• W2912088003 title "Nanostructured Bifunctional Hydrogels as Potential Instructing Platform for Hematopoietic Stem Cell Differentiation" @default.
• W2912088003 cites W1565481082 @default.
• W2912088003 cites W1566413262 @default.
• W2912088003 cites W1816761971 @default.
• W2912088003 cites W1836124358 @default.
• W2912088003 cites W1866239999 @default.
• W2912088003 cites W1968463185 @default.
• W2912088003 cites W1973100852 @default.
• W2912088003 cites W1978363318 @default.
• W2912088003 cites W1981226457 @default.
• W2912088003 cites W1981684224 @default.
• W2912088003 cites W1982970639 @default.
• W2912088003 cites W1983067840 @default.
• W2912088003 cites W1984189217 @default.
• W2912088003 cites W1991424279 @default.
• W2912088003 cites W1993721428 @default.
• W2912088003 cites W1994793050 @default.
• W2912088003 cites W1996519671 @default.
• W2912088003 cites W1997451431 @default.
• W2912088003 cites W1998990074 @default.
• W2912088003 cites W1999848701 @default.
• W2912088003 cites W2000809028 @default.
• W2912088003 cites W2001804869 @default.
• W2912088003 cites W2001909877 @default.
• W2912088003 cites W2006728006 @default.
• W2912088003 cites W2008344299 @default.
• W2912088003 cites W2014422435 @default.
• W2912088003 cites W2015216415 @default.
• W2912088003 cites W2022969625 @default.
• W2912088003 cites W2023282510 @default.
• W2912088003 cites W2028735719 @default.
• W2912088003 cites W2028860972 @default.
• W2912088003 cites W2029007679 @default.
• W2912088003 cites W2029159327 @default.
• W2912088003 cites W2029780765 @default.
• W2912088003 cites W2035244655 @default.
• W2912088003 cites W2036863307 @default.
• W2912088003 cites W2045461231 @default.
• W2912088003 cites W2049953323 @default.
• W2912088003 cites W2050700188 @default.
• W2912088003 cites W2051262064 @default.
• W2912088003 cites W2051292725 @default.
• W2912088003 cites W2052306889 @default.
• W2912088003 cites W2052605694 @default.
• W2912088003 cites W2056598046 @default.
• W2912088003 cites W2058746677 @default.
• W2912088003 cites W2067131315 @default.
• W2912088003 cites W2071980761 @default.
• W2912088003 cites W2072837518 @default.
• W2912088003 cites W2074130081 @default.
• W2912088003 cites W2078494709 @default.
• W2912088003 cites W2079762668 @default.
• W2912088003 cites W2084610464 @default.
• W2912088003 cites W2087760361 @default.
• W2912088003 cites W2089274280 @default.
• W2912088003 cites W2090033479 @default.
• W2912088003 cites W2093050394 @default.
• W2912088003 cites W2093334921 @default.
• W2912088003 cites W2100035392 @default.
• W2912088003 cites W2101244168 @default.
• W2912088003 cites W2106861692 @default.
• W2912088003 cites W2111653667 @default.
• W2912088003 cites W2111732478 @default.
• W2912088003 cites W2115840354 @default.
• W2912088003 cites W2116818706 @default.
• W2912088003 cites W2120908143 @default.
• W2912088003 cites W2124295081 @default.
• W2912088003 cites W2125547274 @default.
• W2912088003 cites W2127080762 @default.
• W2912088003 cites W2134705919 @default.
• W2912088003 cites W2135376666 @default.
• W2912088003 cites W2145708130 @default.
• W2912088003 cites W2147195750 @default.
• W2912088003 cites W2155696890 @default.
• W2912088003 cites W2157979692 @default.
• W2912088003 cites W2161549992 @default.
• W2912088003 cites W2161913720 @default.
• W2912088003 cites W2164312554 @default.
• W2912088003 cites W2164517878 @default.
• W2912088003 cites W2169623356 @default.
• W2912088003 cites W2169683728 @default.
• W2912088003 cites W2399615758 @default.
• W2912088003 cites W2404234352 @default.
• W2912088003 cites W2501931932 @default.
• W2912088003 cites W2569572387 @default.
• W2912088003 cites W2597226366 @default.
• W2912088003 cites W2604331373 @default.
• W2912088003 cites W2604863211 @default.
• W2912088003 cites W2605463821 @default.
• W2912088003 cites W2805908425 @default.
• W2912088003 cites W2864975369 @default.

• next