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• W2912091260 abstract "SUMMARY Large-scale single-cell RNA-sequencing (scRNA-seq) studies that profile hundreds of thousands of cells are becoming increasingly common, overwhelming existing analysis pipelines. Here, we describe how to enhance and accelerate single-cell data analysis by summarizing the transcriptomic heterogeneity within a data set using a small subset of cells, which we refer to as a geometric sketch. Our sketches provide more comprehensive visualization of transcriptional diversity, capture rare cell types with high sensitivity, and accurately reveal biological cell types via clustering. Our sketch of umbilical cord blood cells uncovers a rare subpopulation of inflammatory macrophages, which we experimentally validated in vitro . The construction of our sketches is extremely fast, which enabled us to accelerate other crucial resource-intensive tasks such as scRNA-seq data integration. We anticipate that our algorithm will become an increasingly essential step when sharing and analyzing the rapidly-growing volume of scRNA-seq data and help enable the democratization of single-cell omics." @default.
• W2912091260 created "2019-02-21" @default.
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• W2912091260 date "2019-01-31" @default.
• W2912091260 modified "2023-09-26" @default.
• W2912091260 title "Geometric Sketching Compactly Summarizes the Single-Cell Transcriptomic Landscape" @default.
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• W2912091260 doi "https://doi.org/10.1101/536730" @default.
• W2912091260 hasPublicationYear "2019" @default.
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