FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912091476> ?p ?o ?g. }

• W2912091476 abstract "Genetic modification of plant cell walls has been implemented to reduce lignocellulosic recalcitrance for biofuel production. Plant glycoside hydrolase family 9 (GH9) comprises endo-β-1,4-glucanase in plants. Few studies have examined the roles of GH9 in cell wall modification. In this study, we independently overexpressed two genes from GH9B subclasses (OsGH9B1 and OsGH9B3) and examined cell wall features and biomass saccharification in transgenic rice plants.Compared with the wild type (WT, Nipponbare), the OsGH9B1 and OsGH9B3 transgenic rice plants, respectively, contained much higher OsGH9B1 and OsGH9B3 protein levels and both proteins were observed in situ with nonspecific distribution in the plant cells. The transgenic lines exhibited significantly increased cellulase activity in vitro than the WT. The OsGH9B1 and OsGH9B3 transgenic plants showed a slight alteration in three wall polymer compositions (cellulose, hemicelluloses, and lignin), in their stem mechanical strength and biomass yield, but were significantly decreased in the cellulose degree of polymerization (DP) and lignocellulose crystalline index (CrI) by 21-22%. Notably, the crude cellulose substrates of the transgenic lines were more efficiently digested by cellobiohydrolase (CBHI) than those of the WT, indicating the significantly increased amounts of reducing ends of β-1,4-glucans in cellulose microfibrils. Finally, the engineered lines generated high sugar yields after mild alkali pretreatments and subsequent enzymatic hydrolysis, resulting in the high bioethanol yields obtained at 22.5% of dry matter.Overproduction of OsGH9B1/B3 enzymes should have specific activity in the postmodification of cellulose microfibrils. The increased reducing ends of β-1,4-glucan chains for reduced cellulose DP and CrI positively affected biomass enzymatic saccharification. Our results demonstrate a potential strategy for genetic modification of cellulose microfibrils in bioenergy crops." @default.
• W2912091476 created "2019-02-21" @default.
• W2912091476 creator A5008012096 @default.
• W2912091476 creator A5012102785 @default.
• W2912091476 creator A5017910487 @default.
• W2912091476 creator A5026710935 @default.
• W2912091476 creator A5032423839 @default.
• W2912091476 creator A5052570866 @default.
• W2912091476 creator A5057951077 @default.
• W2912091476 creator A5059283624 @default.
• W2912091476 creator A5060002817 @default.
• W2912091476 creator A5065236658 @default.
• W2912091476 creator A5066257606 @default.
• W2912091476 creator A5074160086 @default.
• W2912091476 date "2019-01-09" @default.
• W2912091476 modified "2023-10-09" @default.
• W2912091476 title "Overproduction of native endo-β-1,4-glucanases leads to largely enhanced biomass saccharification and bioethanol production by specific modification of cellulose features in transgenic rice" @default.
• W2912091476 cites W1534406401 @default.
• W2912091476 cites W1539867889 @default.
• W2912091476 cites W1682922962 @default.
• W2912091476 cites W1940869987 @default.
• W2912091476 cites W1963610135 @default.
• W2912091476 cites W1982267818 @default.
• W2912091476 cites W1982318239 @default.
• W2912091476 cites W1996275966 @default.
• W2912091476 cites W2001396309 @default.
• W2912091476 cites W2003029279 @default.
• W2912091476 cites W2005146231 @default.
• W2912091476 cites W2006531119 @default.
• W2912091476 cites W2014698197 @default.
• W2912091476 cites W2016429076 @default.
• W2912091476 cites W2020648116 @default.
• W2912091476 cites W2022332039 @default.
• W2912091476 cites W2026496523 @default.
• W2912091476 cites W2047760234 @default.
• W2912091476 cites W2052821301 @default.
• W2912091476 cites W2055781489 @default.
• W2912091476 cites W2058867396 @default.
• W2912091476 cites W2059430314 @default.
• W2912091476 cites W2063880652 @default.
• W2912091476 cites W2068314688 @default.
• W2912091476 cites W2072805423 @default.
• W2912091476 cites W2083945613 @default.
• W2912091476 cites W2089058731 @default.
• W2912091476 cites W2091219266 @default.
• W2912091476 cites W2092566243 @default.
• W2912091476 cites W2095900397 @default.
• W2912091476 cites W2096428294 @default.
• W2912091476 cites W2096991082 @default.
• W2912091476 cites W2097706568 @default.
• W2912091476 cites W2108062133 @default.
• W2912091476 cites W2109587007 @default.
• W2912091476 cites W2111335508 @default.
• W2912091476 cites W2113964973 @default.
• W2912091476 cites W2125716499 @default.
• W2912091476 cites W2128056890 @default.
• W2912091476 cites W2128635872 @default.
• W2912091476 cites W2132180631 @default.
• W2912091476 cites W2137641040 @default.
• W2912091476 cites W2139698001 @default.
• W2912091476 cites W2139893028 @default.
• W2912091476 cites W2143310104 @default.
• W2912091476 cites W2143728833 @default.
• W2912091476 cites W2144335345 @default.
• W2912091476 cites W2144383835 @default.
• W2912091476 cites W2152207030 @default.
• W2912091476 cites W2152493712 @default.
• W2912091476 cites W2153487187 @default.
• W2912091476 cites W2159413760 @default.
• W2912091476 cites W2165267802 @default.
• W2912091476 cites W2167554145 @default.
• W2912091476 cites W2168128200 @default.
• W2912091476 cites W2218641619 @default.
• W2912091476 cites W2346563040 @default.
• W2912091476 cites W2581223712 @default.
• W2912091476 cites W2620879269 @default.
• W2912091476 cites W2705275707 @default.
• W2912091476 cites W2754488872 @default.
• W2912091476 cites W2773234480 @default.
• W2912091476 cites W2789949259 @default.
• W2912091476 cites W4244604719 @default.
• W2912091476 cites W4293247451 @default.
• W2912091476 cites W612828076 @default.
• W2912091476 doi "https://doi.org/10.1186/s13068-018-1351-1" @default.
• W2912091476 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6325865" @default.
• W2912091476 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30636971" @default.
• W2912091476 hasPublicationYear "2019" @default.
• W2912091476 type Work @default.
• W2912091476 sameAs 2912091476 @default.
• W2912091476 citedByCount "64" @default.
• W2912091476 countsByYear W29120914762019 @default.
• W2912091476 countsByYear W29120914762020 @default.
• W2912091476 countsByYear W29120914762021 @default.
• W2912091476 countsByYear W29120914762022 @default.
• W2912091476 countsByYear W29120914762023 @default.
• W2912091476 crossrefType "journal-article" @default.
• W2912091476 hasAuthorship W2912091476A5008012096 @default.
• W2912091476 hasAuthorship W2912091476A5012102785 @default.
• W2912091476 hasAuthorship W2912091476A5017910487 @default.
• W2912091476 hasAuthorship W2912091476A5026710935 @default.

• next