Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912125909> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2912125909 endingPage "106" @default.
- W2912125909 startingPage "106" @default.
- W2912125909 abstract "Purpose: HEV is transmitted to humans through the faecal–oral route, mainly by the consumption of contaminated water or undercooked meat from infected animals. Diagnosis of acute hepatitis E infection is based on the detection of specific IgM antibodies to the virus in serum. Additional tests include RT-PCR to detect the HEV RNA. The IgG detection is used to define prior contact and sometimes to confirm seroconversion, for follow-up of acute cases. The aim of this study is the assessment of a new serological method that focuses in sensitivity and it is compared to other methods previously assessed. Methods & Materials: Samples have been processed in VIRCLIA equipment of Chemiluminescent CLIA (HEV based on the capture of IgM present in the sample by antiinmunoglobuline antibodies and IgG, by antigens attached to the surface of polystyrene. Two different panels have been configured:IgM Panel: 52 samples including 13 serum samples from patients with acute hepatitis and genotype 3 virus infection (confirmed by Immunoblot and molecular methods) and their serial dilutions 1:10, 1:100 and 1:1000.IgG Panel: 40 samples including 10 serum samples from seroconversion-phase patients after acute infection and their serial dilutions 1:10, 1:100 and 1:1000. These panels have been used to evaluate other screening methods (Wantai Biological Pharmacy, Euro-Immun Medizinische, MP biomedicals, Dia.Pro diagnostics bioprobes, DSI S.R.L., Saronno, Italy. Mikrogen GmbH Neuried and HEVTOP All Diag), which results have already been published. Results: Anti HEV-IgM: All tested specimens had positive results in undiluted specimens, at 1:10, and at 1:100. Of all samples 77% were positive (40/52). Anti HEV-IgG: All tested specimens had positive results in undiluted samples and dilutions 1:10 and 7 of them also reached the 1:100 dilution. Of all samples 77.5% were positive (31/40). Conclusion: Compared to other methods The HEV-IgM VirClia® System has greater sensitivity and it is only exceeded by the DSI system. The HEV-IgG VirClia® System has greater sensitivity with the exception of the Dia.pro system, which has the same sensitivity. The Chemiluminescence VIRCLIA® method is presented as a reliable serological method for the screening of acute infection by HEV in clinical specimens." @default.
- W2912125909 created "2019-02-21" @default.
- W2912125909 creator A5002524259 @default.
- W2912125909 creator A5019826487 @default.
- W2912125909 creator A5030682643 @default.
- W2912125909 creator A5049895776 @default.
- W2912125909 creator A5059235047 @default.
- W2912125909 creator A5075023055 @default.
- W2912125909 date "2019-02-01" @default.
- W2912125909 modified "2023-10-17" @default.
- W2912125909 title "Assessment of the hepatitis E Virclia® system for the detection of IgM and IgG antibodies against HEV" @default.
- W2912125909 doi "https://doi.org/10.1016/j.ijid.2018.11.263" @default.
- W2912125909 hasPublicationYear "2019" @default.
- W2912125909 type Work @default.
- W2912125909 sameAs 2912125909 @default.
- W2912125909 citedByCount "0" @default.
- W2912125909 crossrefType "journal-article" @default.
- W2912125909 hasAuthorship W2912125909A5002524259 @default.
- W2912125909 hasAuthorship W2912125909A5019826487 @default.
- W2912125909 hasAuthorship W2912125909A5030682643 @default.
- W2912125909 hasAuthorship W2912125909A5049895776 @default.
- W2912125909 hasAuthorship W2912125909A5059235047 @default.
- W2912125909 hasAuthorship W2912125909A5075023055 @default.
- W2912125909 hasBestOaLocation W29121259091 @default.
- W2912125909 hasConcept C104317684 @default.
- W2912125909 hasConcept C135763542 @default.
- W2912125909 hasConcept C142724271 @default.
- W2912125909 hasConcept C159047783 @default.
- W2912125909 hasConcept C159654299 @default.
- W2912125909 hasConcept C184112429 @default.
- W2912125909 hasConcept C203014093 @default.
- W2912125909 hasConcept C204787440 @default.
- W2912125909 hasConcept C2776553844 @default.
- W2912125909 hasConcept C2777451964 @default.
- W2912125909 hasConcept C2780777367 @default.
- W2912125909 hasConcept C2780898057 @default.
- W2912125909 hasConcept C45189115 @default.
- W2912125909 hasConcept C55493867 @default.
- W2912125909 hasConcept C71924100 @default.
- W2912125909 hasConcept C86803240 @default.
- W2912125909 hasConceptScore W2912125909C104317684 @default.
- W2912125909 hasConceptScore W2912125909C135763542 @default.
- W2912125909 hasConceptScore W2912125909C142724271 @default.
- W2912125909 hasConceptScore W2912125909C159047783 @default.
- W2912125909 hasConceptScore W2912125909C159654299 @default.
- W2912125909 hasConceptScore W2912125909C184112429 @default.
- W2912125909 hasConceptScore W2912125909C203014093 @default.
- W2912125909 hasConceptScore W2912125909C204787440 @default.
- W2912125909 hasConceptScore W2912125909C2776553844 @default.
- W2912125909 hasConceptScore W2912125909C2777451964 @default.
- W2912125909 hasConceptScore W2912125909C2780777367 @default.
- W2912125909 hasConceptScore W2912125909C2780898057 @default.
- W2912125909 hasConceptScore W2912125909C45189115 @default.
- W2912125909 hasConceptScore W2912125909C55493867 @default.
- W2912125909 hasConceptScore W2912125909C71924100 @default.
- W2912125909 hasConceptScore W2912125909C86803240 @default.
- W2912125909 hasLocation W29121259091 @default.
- W2912125909 hasOpenAccess W2912125909 @default.
- W2912125909 hasPrimaryLocation W29121259091 @default.
- W2912125909 hasRelatedWork W177744588 @default.
- W2912125909 hasRelatedWork W2094203884 @default.
- W2912125909 hasRelatedWork W2105066020 @default.
- W2912125909 hasRelatedWork W2125178191 @default.
- W2912125909 hasRelatedWork W2421028750 @default.
- W2912125909 hasRelatedWork W2510872658 @default.
- W2912125909 hasRelatedWork W2948555349 @default.
- W2912125909 hasRelatedWork W3039456144 @default.
- W2912125909 hasRelatedWork W3127359265 @default.
- W2912125909 hasRelatedWork W3128830683 @default.
- W2912125909 hasVolume "79" @default.
- W2912125909 isParatext "false" @default.
- W2912125909 isRetracted "false" @default.
- W2912125909 magId "2912125909" @default.
- W2912125909 workType "article" @default.