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- W2912126732 abstract "Abstract Non-viral gene carriers have been extensively investigated as alternatives to viral vectors for gene delivery systems into animal and plant cells. A non-viral gene carrier containing a cell-penetrating peptide and a cationic sequence was previously developed for use in intact plants and plant cells; however, the permeation pathway of the gene carrier into plant cells is yet to be elucidated, which would facilitate the improvement of the gene delivery efficiency. Here, we identified the vacuum/compression infiltration-mediated permeation pathway of a non-viral gene carrier into plant tissues and cells using a complex of plasmid DNA and a peptide-based gene carrier. This complex was taken up via the hydathodes in Arabidopsis thaliana , and from root hairs in Nicotiana benthamiana . Remarkably, these structurally weak tissues are also routes of bacterial invasion in nature, suggesting that peptide-pDNA complexes invade intact plants through similar pathways as bacterial pathogens." @default.
- W2912126732 created "2019-02-21" @default.
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- W2912126732 date "2019-01-22" @default.
- W2912126732 modified "2023-10-12" @default.
- W2912126732 title "Vacuum/Compression Infiltration-mediated Permeation Pathway of a Peptide-pDNA Complex as a Non-Viral Carrier for Gene Delivery in Planta" @default.
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- W2912126732 doi "https://doi.org/10.1038/s41598-018-36466-1" @default.
- W2912126732 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6342927" @default.
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