Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912132694> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2912132694 abstract "Dominant-negative (DN) protein inhibition is a powerful method to manipulate protein function and offers several advantages over other genome-based approaches. For example, although chimeric and Cre-LoxP targeting strategies have been widely used, the intrinsic limitations of these strategies (i.e., leaky promoter activity, mosaic Cre expression, etc.) have significantly restricted their application. Moreover, a complete deletion of many endogenous genes is embryonically lethal, making it impossible to study gene function in postnatal life. To address these challenges, we have made significant changes to an early genetic engineering protocol and combined a short (transgenic) version of the Rb1 gene with a lysosomal protease procathepsin B (CB), to generate a DN mouse model of Rb1 (CBRb). Due to the presence of a lysosomal protease, the entire CB-RB1 fusion protein and its interacting complex are routed for proteasome-mediated degradation. Moreover, the presence of a tetracycline inducer (rtTA) element in the transgenic construct enables an inducible and reversible regulation of the RB1 protein. The presence of a ubiquitous ROSA-CAG promoter in the CBRb mouse model makes it a useful tool to carry out transient and reversible Rb1 gene ablation and provide researchers a resource for understanding its activity in virtually any cell type where RB1 is expressed." @default.
- W2912132694 created "2019-02-21" @default.
- W2912132694 creator A5000716568 @default.
- W2912132694 creator A5024623066 @default.
- W2912132694 creator A5051873208 @default.
- W2912132694 creator A5055765316 @default.
- W2912132694 creator A5091753415 @default.
- W2912132694 date "2019-01-07" @default.
- W2912132694 modified "2023-09-25" @default.
- W2912132694 title "Inducible and Reversible Dominant-negative (DN) Protein Inhibition" @default.
- W2912132694 doi "https://doi.org/10.3791/58419" @default.
- W2912132694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30663696" @default.
- W2912132694 hasPublicationYear "2019" @default.
- W2912132694 type Work @default.
- W2912132694 sameAs 2912132694 @default.
- W2912132694 citedByCount "0" @default.
- W2912132694 crossrefType "journal-article" @default.
- W2912132694 hasAuthorship W2912132694A5000716568 @default.
- W2912132694 hasAuthorship W2912132694A5024623066 @default.
- W2912132694 hasAuthorship W2912132694A5051873208 @default.
- W2912132694 hasAuthorship W2912132694A5055765316 @default.
- W2912132694 hasAuthorship W2912132694A5091753415 @default.
- W2912132694 hasConcept C102230213 @default.
- W2912132694 hasConcept C104317684 @default.
- W2912132694 hasConcept C111829193 @default.
- W2912132694 hasConcept C123894998 @default.
- W2912132694 hasConcept C153911025 @default.
- W2912132694 hasConcept C181199279 @default.
- W2912132694 hasConcept C2776714187 @default.
- W2912132694 hasConcept C40767141 @default.
- W2912132694 hasConcept C54355233 @default.
- W2912132694 hasConcept C55493867 @default.
- W2912132694 hasConcept C86803240 @default.
- W2912132694 hasConcept C95444343 @default.
- W2912132694 hasConceptScore W2912132694C102230213 @default.
- W2912132694 hasConceptScore W2912132694C104317684 @default.
- W2912132694 hasConceptScore W2912132694C111829193 @default.
- W2912132694 hasConceptScore W2912132694C123894998 @default.
- W2912132694 hasConceptScore W2912132694C153911025 @default.
- W2912132694 hasConceptScore W2912132694C181199279 @default.
- W2912132694 hasConceptScore W2912132694C2776714187 @default.
- W2912132694 hasConceptScore W2912132694C40767141 @default.
- W2912132694 hasConceptScore W2912132694C54355233 @default.
- W2912132694 hasConceptScore W2912132694C55493867 @default.
- W2912132694 hasConceptScore W2912132694C86803240 @default.
- W2912132694 hasConceptScore W2912132694C95444343 @default.
- W2912132694 hasIssue "143" @default.
- W2912132694 hasLocation W29121326941 @default.
- W2912132694 hasLocation W29121326942 @default.
- W2912132694 hasOpenAccess W2912132694 @default.
- W2912132694 hasPrimaryLocation W29121326941 @default.
- W2912132694 hasRelatedWork W2355216131 @default.
- W2912132694 hasRelatedWork W2363228671 @default.
- W2912132694 hasRelatedWork W2363858492 @default.
- W2912132694 hasRelatedWork W2365121371 @default.
- W2912132694 hasRelatedWork W2367069379 @default.
- W2912132694 hasRelatedWork W2388046372 @default.
- W2912132694 hasRelatedWork W2393451491 @default.
- W2912132694 hasRelatedWork W2465424684 @default.
- W2912132694 hasRelatedWork W2973460871 @default.
- W2912132694 hasRelatedWork W3031625311 @default.
- W2912132694 isParatext "false" @default.
- W2912132694 isRetracted "false" @default.
- W2912132694 magId "2912132694" @default.
- W2912132694 workType "article" @default.