Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912132968> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2912132968 endingPage "110a" @default.
- W2912132968 startingPage "110a" @default.
- W2912132968 abstract "Members of the ENaC/DEG superfamily of ion channels are Na+-selective ion channels with a common trimeric architecture. Each of the three subunits has two transmembrane helices (M1 and M2), of which M2 lines the pore. Members of this family include the acid-sensing ion channels (ASICs), formed by identical or homologous subunits that mediate excitatory Na+ currents in the nervous system (relative Na+/K+ permeability of approx. 10/1). The epithelial sodium channels (ENaCs) are obligate heterotrimers and display a 10-fold higher Na+ selectivity than ASICs. The most recent findings indicate that this discrepancy might be due to different selectivity filter (SF) locations; while the ENaC SF is likely formed by the conserved G/S-X-S motif in the center of the pore, we have recently shown the mouse ASIC1a SF to be composed of two acidic side chains in the lower part of M2, namely E18’ and D21’. In order to elucidate if other parts of the channel contribute to the stark differences in Na+ selectivity between ASICs and ENaCs, we used conventional and non-canonical amino acid substitutions to probe the contribution of M1 residues to ion selectivity. Our results show that aromatic residues in ASIC M1 are important for ion selectivity. ENaC contains additional aromatic residues in M1. We hypothesize that these aromatics are similarly important for ion selectivity and that pore diameter plays an important role in both channels. Furthermore, the intracellular domains have previously been suggested to contribute to ion selectivity. Using a novel split intein-based approach we fuse partial ASIC1a constructs with recombinant or synthetic peptides corresponding to the N- or C-terminus of the full length protein. This enables us to introduce non-canonical amino acid substitutions, including post-translational modifications, into these less-studied regions of the channel." @default.
- W2912132968 created "2019-02-21" @default.
- W2912132968 creator A5045259945 @default.
- W2912132968 creator A5065242834 @default.
- W2912132968 creator A5074752967 @default.
- W2912132968 creator A5089788338 @default.
- W2912132968 date "2019-02-01" @default.
- W2912132968 modified "2023-10-14" @default.
- W2912132968 title "Indirect Determinants of Ion Selectivity in Acid-Sensing Ion Channels and Epithelial Sodium Channels" @default.
- W2912132968 doi "https://doi.org/10.1016/j.bpj.2018.11.626" @default.
- W2912132968 hasPublicationYear "2019" @default.
- W2912132968 type Work @default.
- W2912132968 sameAs 2912132968 @default.
- W2912132968 citedByCount "0" @default.
- W2912132968 crossrefType "journal-article" @default.
- W2912132968 hasAuthorship W2912132968A5045259945 @default.
- W2912132968 hasAuthorship W2912132968A5065242834 @default.
- W2912132968 hasAuthorship W2912132968A5074752967 @default.
- W2912132968 hasAuthorship W2912132968A5089788338 @default.
- W2912132968 hasBestOaLocation W29121329681 @default.
- W2912132968 hasConcept C118792377 @default.
- W2912132968 hasConcept C118892022 @default.
- W2912132968 hasConcept C12554922 @default.
- W2912132968 hasConcept C143928317 @default.
- W2912132968 hasConcept C145148216 @default.
- W2912132968 hasConcept C161790260 @default.
- W2912132968 hasConcept C170493617 @default.
- W2912132968 hasConcept C178790620 @default.
- W2912132968 hasConcept C185592680 @default.
- W2912132968 hasConcept C41625074 @default.
- W2912132968 hasConcept C45516822 @default.
- W2912132968 hasConcept C50254741 @default.
- W2912132968 hasConcept C50952357 @default.
- W2912132968 hasConcept C515207424 @default.
- W2912132968 hasConcept C537181965 @default.
- W2912132968 hasConcept C55493867 @default.
- W2912132968 hasConcept C6507245 @default.
- W2912132968 hasConcept C86803240 @default.
- W2912132968 hasConcept C99975950 @default.
- W2912132968 hasConceptScore W2912132968C118792377 @default.
- W2912132968 hasConceptScore W2912132968C118892022 @default.
- W2912132968 hasConceptScore W2912132968C12554922 @default.
- W2912132968 hasConceptScore W2912132968C143928317 @default.
- W2912132968 hasConceptScore W2912132968C145148216 @default.
- W2912132968 hasConceptScore W2912132968C161790260 @default.
- W2912132968 hasConceptScore W2912132968C170493617 @default.
- W2912132968 hasConceptScore W2912132968C178790620 @default.
- W2912132968 hasConceptScore W2912132968C185592680 @default.
- W2912132968 hasConceptScore W2912132968C41625074 @default.
- W2912132968 hasConceptScore W2912132968C45516822 @default.
- W2912132968 hasConceptScore W2912132968C50254741 @default.
- W2912132968 hasConceptScore W2912132968C50952357 @default.
- W2912132968 hasConceptScore W2912132968C515207424 @default.
- W2912132968 hasConceptScore W2912132968C537181965 @default.
- W2912132968 hasConceptScore W2912132968C55493867 @default.
- W2912132968 hasConceptScore W2912132968C6507245 @default.
- W2912132968 hasConceptScore W2912132968C86803240 @default.
- W2912132968 hasConceptScore W2912132968C99975950 @default.
- W2912132968 hasIssue "3" @default.
- W2912132968 hasLocation W29121329681 @default.
- W2912132968 hasLocation W29121329682 @default.
- W2912132968 hasOpenAccess W2912132968 @default.
- W2912132968 hasPrimaryLocation W29121329681 @default.
- W2912132968 hasRelatedWork W1616840764 @default.
- W2912132968 hasRelatedWork W2051237359 @default.
- W2912132968 hasRelatedWork W2127721177 @default.
- W2912132968 hasRelatedWork W2513576822 @default.
- W2912132968 hasRelatedWork W2785993069 @default.
- W2912132968 hasRelatedWork W2787634327 @default.
- W2912132968 hasRelatedWork W2888191597 @default.
- W2912132968 hasRelatedWork W3096563727 @default.
- W2912132968 hasRelatedWork W3131563630 @default.
- W2912132968 hasRelatedWork W4206061877 @default.
- W2912132968 hasVolume "116" @default.
- W2912132968 isParatext "false" @default.
- W2912132968 isRetracted "false" @default.
- W2912132968 magId "2912132968" @default.
- W2912132968 workType "article" @default.