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• W2912149961 startingPage "54" @default.
• W2912149961 abstract "Atherosclerosis is an inflammatory disease of the arterial wall caused by the formation of an atheroma plaque in the vessel wall. The uptake of modified LDL lipoproteins by sub-endothelial macrophages induces the latter's transformation into foam cells, which is the key step of atheroma plaque formation. The modifications of neutral lipids caused by foam cells formation are marked by the appearance of lipid droplets. Polyunsaturated fatty acids (PUFAs) incorporation into membrane phospholipids (PL) modifies their composition, which may influence membrane protein functions. The incorporation of eicosapentaenoic acid (EPA) reduces the anti-atherogenic ABCA1 (ATP Binding Cassette transporter A1) pathway and induces PLs modifications. In order to study lipids directly in the cell environment, a comparative study is conducted by vibrational spectroscopies on murine macrophages J774, loaded or not with cholesterol, which were enriched or not with eicosapentaenoic acid (EPA). The study enabled to identify changes in the spectral signature after cells enrichment with fatty acid (FA) relying only on chemometric analysis without deuterium labelling. Results highlighted spectral changes in the regions attributed to lipids associated to triglycerides, phospholipids and cholesterol in both Raman and IR." @default.
• W2912149961 created "2019-02-21" @default.
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• W2912149961 date "2019-07-01" @default.
• W2912149961 modified "2023-09-25" @default.
• W2912149961 title "Investigation of lipid modifications in J774 macrophages by vibrational spectroscopies after eicosapentaenoic acid membrane incorporation in unloaded and cholesterol-loaded cells" @default.
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• W2912149961 doi "https://doi.org/10.1016/j.talanta.2019.01.122" @default.
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• W2912149961 hasPublicationYear "2019" @default.
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