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• W2912151422 abstract "Fluorescence imaging in the second near‐infrared optical window (NIR‐II, 900‐1700 nm) has become a technique of choice for noninvasive in vivo imaging in recent years. Greater penetration depths with high spatial resolution and low background can be achieved with this NIR‐II window, owing to low autofluorescence within this optical range and reduced scattering of long wavelength photons. Here, we present a novel design of confocal laser scanning microscope tailored for imaging in the NIR‐II window. We showcase the outstanding penetration depth of our confocal setup with a series of imaging experiments. HeLa cells labeled with PbS quantum dots with a peak emission wavelength of 1276 nm can be visualized through a 3.5‐mm‐thick layer of scattering medium, which is a 0.8% Lipofundin solution. A commercially available organic dye IR‐1061 (emission peak at 1132 nm), in its native form, is used for the first time, as a NIR‐II fluorescence label in cellular imaging. Our confocal setup is capable of capturing optically sectioned images of IR‐1061 labeled chondrocytes in fixed animal cartilage at a depth up to 800 μm, with a superb spatial resolution of around 2 μm." @default.
• W2912151422 created "2019-02-21" @default.
• W2912151422 creator A5009086593 @default.
• W2912151422 creator A5016171481 @default.
• W2912151422 date "2019-04-01" @default.
• W2912151422 modified "2023-10-06" @default.
• W2912151422 title "Three‐dimensional cellular imaging in thick biological tissue with confocal detection of one‐photon fluorescence in the near‐infrared II window" @default.
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• W2912151422 doi "https://doi.org/10.1002/jbio.201800459" @default.
• W2912151422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30663282" @default.
• W2912151422 hasPublicationYear "2019" @default.
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