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- W2912158345 abstract "DNA knots are self-entanglements of long DNA molecules, crucial in key biological processes such as viral DNA packaging, DNA replication, etc. Nanopore nanoscopy is a promising tool that allows for the large-scale sampling of single DNA molecules in solutions. Recently, we demonstrated the co-existence of simple and complex knots with statistical significance using nanopore sensors. Here, using our large sampling of single-molecule events in concert with parameterized simulations, we investigated the complexity of DNA knots and their dependence on several experimental parameters such as ionic concentration, nanopore diameter, etc. Our results showed a surprising increase in the probability and complexity of knotting with increasing ionic concentration, even beyond the theoretical saturation point of persistence length of DNA. In addition, we probed the localization of knots and various modes of translocation as a function of ionic concentration. Our results reveal that equilibrium distributions of knots could be obtained via controlling the sliding of knots by tuning the experimental parameters such as nanopore diameter. These results are useful in understanding the knotting phenomena in elongated polymers as well as in the nanopore based next generation genome sequencing applications. Keywords: Solid-state nanopores, DNA knots, polymers, DNA sequencing, topology Acknowledgement: This work was supported by National Research Foundation, Singapore Grant (Grant No. NRF-NRFF2012-09) and Singapore-MIT Alliance for Research and Technology Centre." @default.
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- W2912158345 date "2019-02-01" @default.
- W2912158345 modified "2023-10-18" @default.
- W2912158345 title "Nanopore Detection of Surprising Molarity Dependence of DNA Knot Complexity" @default.
- W2912158345 doi "https://doi.org/10.1016/j.bpj.2018.11.219" @default.
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