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• W2912169832 abstract "Mesenchymal stromal cell (MSC) therapy has produced very promising results for multiple diseases in animal models, with over 780 clinical trials on going or completed. However, most of the human clinical trials have not been as successful as trials using preclinical models. To improve the therapeutic potential of MSCs, different research groups have used gene transfer vectors to express factors involved in migration, survival, differentiation, and immunomodulation. The ideal gene transfer vector for most applications should achieve long-term, stable (constitutive or inducible) transgene expression in MSCs and their progeny. Given their efficiency and low impact on transduced cells, lentiviral vectors (LVs) are the vectors of choice. In this chapter we will describe a detailed protocol for the generation of genetically modified MSCs using lentiviral vectors (LVs). Although this protocol has been optimized for MSC lentiviral transduction, it can be easily adapted to other stem cells by changing culture conditions while maintaining volumes and incubation times." @default.
• W2912169832 created "2019-02-21" @default.
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• W2912169832 date "2019-01-01" @default.
• W2912169832 modified "2023-09-27" @default.
• W2912169832 title "Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors" @default.
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• W2912169832 doi "https://doi.org/10.1007/978-1-4939-9065-8_17" @default.
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