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• W2912184295 abstract "The development of a feasible antibiotic detection method is important in water quality analysis. In this study, we developed a metal-organic framework (MOF)-aptamer-3,3′,5,5′-tetramethylbenzidine (TMB)-H2O2-based sensing platform composed of the reaction variable of TMB catalytic oxidation as the label (from colorless to blue) and aptamer as the target recognition element for antibiotic detection. The platform works by calculating the relation between the antibiotic concentration and the resultant decrease in MOF's catalytic activity. Basing from the comparison of typical iron-based MOF materials (Fe-MIL-53, Fe-MIL-88A, and Fe-MIL-100), we selected Fe-MIL-53 to obtain an improved signal amplification effect. The outstanding performance of the Fe-MIL-53-based sensing platform can be attributed to its topological flexibility and small electron transfer impedance. In addition, a signal increment of up to 86% was obtained with an intensified gold nanoparticle (AuNP)-supported aptamer. The inhibitory catalytic activity stemmed from the coating of antibiotic-(AuNP-aptamer) conjugates onto the outer surface of the MOF material, which increased the impedance and decreased the electron transfer efficiency. Validation results indicated that the platform showed high selectivity and sensitivity (i.e., wide linearity range of 50–200 nM, detection limit up to 8.1 ng/mL, and recovery rate of 106%–110%) for chloramphenicol detection and universal applicability for other antibiotics, including ampicillin, tetracycline, and oxytetracycline. In general, the detection reliability and easy operation of this platform render it a promising candidate for antibiotic detection in future water quality monitoring practices." @default.
• W2912184295 created "2019-02-21" @default.
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• W2912184295 date "2019-04-01" @default.
• W2912184295 modified "2023-10-17" @default.
• W2912184295 title "Novel sensing platform based on gold nanoparticle-aptamer and Fe-metal-organic framework for multiple antibiotic detection and signal amplification" @default.
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• W2912184295 doi "https://doi.org/10.1016/j.envint.2019.01.033" @default.
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