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- W2912186752 abstract "The emergent properties of the array arrangement of the molecular motor myosin II in the striated muscle are studied with a synthetic one-dimensional nanomachine, in which HMM fragments, purified from fast skeletal muscle, are randomly dispersed on a chemically etched optical fibre and brought to interact with a single actin filament attached to a bead trapped in the focus of a Dual Laser Optical Tweezers (DLOT, Pertici et al Nat. Commun. 9:3532, 2018). The mechanical output of the machine is measured by means of the DLOT, which acts as a force transducer, and a piezoelectric nano-positioner carrying the support for the motors, which acts as a length transducer. The number of HMM available for actin interaction is estimated from the rupture events in rigor. Isometric and isotonic contractions of striated muscle are reproduced by the motor ensemble in 2 mM ATP switching the control from position to force feedback. Following a drop in force from the maximum isometric value (F0) to a lower value (F), the actin filament slides at a constant velocity (V). Up to five F-V points for each interaction can be determined, allowing the definition of the load dependence of the shortening velocity and power. The results are explained with a kinetic model fitted to the performance of mammalian skeletal muscle, showing that the condition for the motor coordination that maximizes the efficiency in striated muscle is a minimum of 32 motors sharing a common mechanical ground. The nanomachine offers an unprecedented tool for investigating muscle contractile-protein physiology, pathology and pharmacology without the confounding effects of the cytoskeletal and regulatory proteins, the role of which can then be selectively tested with different degrees of reconstitution. Supported by IIT-SEED, Genova and Fondazione CR Firenze, Italy." @default.
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- W2912186752 date "2019-02-01" @default.
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- W2912186752 title "A Myosin II Nanomachine Mimicking the Striated Muscle" @default.
- W2912186752 doi "https://doi.org/10.1016/j.bpj.2018.11.081" @default.
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