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• W2912189716 abstract "Abstract Macrophages are phagocytic cells from the innate immune system, which forms the first line of host defense against invading pathogens. These highly dynamic immune cells can adopt specific functional phenotypes, with the pro-inflammatory M1 and anti-inflammatory M2 polarization states as the two extremes. Recently, the process of macrophage polarization during inflammation has been visualized by real time imaging in larvae of the zebrafish. This model organism has also become widely used to study macrophage responses to microbial pathogens. To support the increasing use of zebrafish in macrophage biology, we set out to determine the complete transcriptome of zebrafish larval macrophages. We studied the specificity of the macrophage signature compared with other larval immune cells and the macrophage-specific expression changes upon infection. We made use of the well-established mpeg1 , mpx , and lck fluorescent reporter lines to sort and sequence the transcriptome of larval macrophages, neutrophils, and lymphoid progenitor cells, respectively. Our results provide a complete dataset of genes expressed in these different immune cell types and highlight their similarities and differences. Major differences between the macrophage and neutrophil signatures were found within the families of proteinases. Furthermore, expression of genes involved in antigen presentation and processing was specifically detected in macrophages, while lymphoid progenitors showed expression of genes involved in macrophage activation. Comparison with datasets of in vitro polarized human macrophages revealed that zebrafish macrophages express a strongly homologous gene set, comprising both M1 and M2 markers. Furthermore, transcriptome analysis of low numbers of macrophages infected by the intracellular pathogen Mycobacterium marinum revealed that infected macrophages change their transcriptomic response by downregulation of M2-associated genes and overexpression of specific M1-associated genes. Among the infection-induced genes, a homolog of the human CXCL11 chemokine gene, cxcl11aa , stood out as the most strongly overexpressed M1 marker. Upregulation of cxcl11aa in Mycobacterium -infected macrophages was found to require the function of Myd88, a critical adaptor molecule in the Toll-like and interleukin 1 receptor pathways that are central to pathogen recognition and activation of the innate immune response. Altogether, our data provide a valuable data mining resource to support infection and inflammation research in the zebrafish model." @default.
• W2912189716 created "2019-02-21" @default.
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• W2912189716 date "2019-02-19" @default.
• W2912189716 modified "2023-09-27" @default.
• W2912189716 title "RNAseq profiling of leukocyte populations in zebrafish larvae reveals a <i>cxcl11</i> chemokine gene as a marker of macrophage polarization during mycobacterial infection" @default.
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• W2912189716 cites W1964578013 @default.
• W2912189716 cites W1967818547 @default.
• W2912189716 cites W1973893754 @default.
• W2912189716 cites W1974375799 @default.
• W2912189716 cites W1980353886 @default.
• W2912189716 cites W1990484548 @default.
• W2912189716 cites W1994942139 @default.
• W2912189716 cites W2002047195 @default.
• W2912189716 cites W2020179710 @default.
• W2912189716 cites W2026016063 @default.
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• W2912189716 cites W2055700705 @default.
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• W2912189716 cites W2064964564 @default.
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• W2912189716 cites W2085432374 @default.
• W2912189716 cites W2086227247 @default.
• W2912189716 cites W2095622948 @default.
• W2912189716 cites W2096173332 @default.
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• W2912189716 cites W2123679840 @default.
• W2912189716 cites W2124289914 @default.
• W2912189716 cites W2130410032 @default.
• W2912189716 cites W2134083365 @default.
• W2912189716 cites W2134690112 @default.
• W2912189716 cites W2138948319 @default.
• W2912189716 cites W2143540026 @default.
• W2912189716 cites W2144639095 @default.
• W2912189716 cites W2153559789 @default.
• W2912189716 cites W2156420692 @default.
• W2912189716 cites W2158014788 @default.
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• W2912189716 cites W2164474259 @default.
• W2912189716 cites W2418747985 @default.
• W2912189716 cites W2479197761 @default.
• W2912189716 cites W2516344353 @default.
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• W2912189716 cites W2572647583 @default.
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• W2912189716 doi "https://doi.org/10.1101/554808" @default.
• W2912189716 hasPublicationYear "2019" @default.
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