Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912195566> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2912195566 abstract "Restriction-modification (RM) represents the simplest and possibly the most widespread mechanism of self/non-self discrimination in nature. In order to provide bacteria with immunity against bacteriophages and other parasitic genetic elements, RM systems rely on a balance between two enzymes: the restriction enzyme, which cleaves non-self DNA at specific restriction sites, and the modification enzyme, which tags the host’s DNA as self and thus protects it from cleavage. In this thesis, I use population and single-cell level experiments in combination with mathematical modeling to study different aspects of the interplay between RM systems, bacteria and bacteriophages. First, I analyze how mutations in phage restriction sites affect the probability of phage escape – an inherently stochastic process, during which phages accidently get modified instead of restricted. Next, I use single-cell experiments to show that RM systems can, with a low probability, attack the genome of their bacterial host and that this primitive form of autoimmunity leads to a tradeoff between the evolutionary cost and benefit of RM systems. Finally, I investigate the nature of interactions between bacteria, RM systems and temperate bacteriophages to find that, as a consequence of phage escape and its impact on population dynamics, RM systems can promote acquisition of symbiotic bacteriophages, rather than limit it. The results presented here uncover new fundamental biological properties of RM systems and highlight their importance in the ecology and evolution of bacteria, bacteriophages and their interactions." @default.
- W2912195566 created "2019-02-21" @default.
- W2912195566 creator A5042306442 @default.
- W2912195566 date "2017-01-01" @default.
- W2912195566 modified "2023-09-27" @default.
- W2912195566 title "Biology of restriction-modification systems at the single-cell and population level" @default.
- W2912195566 hasPublicationYear "2017" @default.
- W2912195566 type Work @default.
- W2912195566 sameAs 2912195566 @default.
- W2912195566 citedByCount "0" @default.
- W2912195566 crossrefType "dissertation" @default.
- W2912195566 hasAuthorship W2912195566A5042306442 @default.
- W2912195566 hasConcept C104317684 @default.
- W2912195566 hasConcept C128319531 @default.
- W2912195566 hasConcept C141231307 @default.
- W2912195566 hasConcept C144024400 @default.
- W2912195566 hasConcept C149923435 @default.
- W2912195566 hasConcept C191908910 @default.
- W2912195566 hasConcept C2908647359 @default.
- W2912195566 hasConcept C54355233 @default.
- W2912195566 hasConcept C552990157 @default.
- W2912195566 hasConcept C70721500 @default.
- W2912195566 hasConcept C86803240 @default.
- W2912195566 hasConceptScore W2912195566C104317684 @default.
- W2912195566 hasConceptScore W2912195566C128319531 @default.
- W2912195566 hasConceptScore W2912195566C141231307 @default.
- W2912195566 hasConceptScore W2912195566C144024400 @default.
- W2912195566 hasConceptScore W2912195566C149923435 @default.
- W2912195566 hasConceptScore W2912195566C191908910 @default.
- W2912195566 hasConceptScore W2912195566C2908647359 @default.
- W2912195566 hasConceptScore W2912195566C54355233 @default.
- W2912195566 hasConceptScore W2912195566C552990157 @default.
- W2912195566 hasConceptScore W2912195566C70721500 @default.
- W2912195566 hasConceptScore W2912195566C86803240 @default.
- W2912195566 hasLocation W29121955661 @default.
- W2912195566 hasOpenAccess W2912195566 @default.
- W2912195566 hasPrimaryLocation W29121955661 @default.
- W2912195566 hasRelatedWork W2015764680 @default.
- W2912195566 hasRelatedWork W2028178639 @default.
- W2912195566 hasRelatedWork W2055432250 @default.
- W2912195566 hasRelatedWork W2057265554 @default.
- W2912195566 hasRelatedWork W2098571831 @default.
- W2912195566 hasRelatedWork W2121998750 @default.
- W2912195566 hasRelatedWork W2123675906 @default.
- W2912195566 hasRelatedWork W2124420137 @default.
- W2912195566 hasRelatedWork W2587590578 @default.
- W2912195566 hasRelatedWork W2739051152 @default.
- W2912195566 hasRelatedWork W2941517942 @default.
- W2912195566 hasRelatedWork W2963188901 @default.
- W2912195566 hasRelatedWork W2964289852 @default.
- W2912195566 hasRelatedWork W2974798315 @default.
- W2912195566 hasRelatedWork W2987852638 @default.
- W2912195566 hasRelatedWork W3041227730 @default.
- W2912195566 hasRelatedWork W3100739105 @default.
- W2912195566 hasRelatedWork W3121471268 @default.
- W2912195566 hasRelatedWork W3164313689 @default.
- W2912195566 hasRelatedWork W2399771462 @default.
- W2912195566 isParatext "false" @default.
- W2912195566 isRetracted "false" @default.
- W2912195566 magId "2912195566" @default.
- W2912195566 workType "dissertation" @default.