FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912204111> ?p ?o ?g. }

• W2912204111 endingPage "e0211961" @default.
• W2912204111 startingPage "e0211961" @default.
• W2912204111 abstract "Background Interaction of von Willebrand factor (VWF) with platelets requires a conformational change that exposes an epitope within the VWF A1 domain, enabling platelet glycoprotein Ibα binding. Quantification of this ‘‘active” conformation of VWF has been shown to provide pathophysiological insight into conditions characterized by excessive VWF-platelet interaction. Methods We developed an immunosorbent assay based on a variable heavy chain antibody fragment against the VWF A1 domain as a capture antibody. Assay performance in terms of specificity (binding to active R1306W- and sheared VWF), precision, accuracy, linearity, limits of detection and stability were determined. Active VWF, VWF antigen, VWF ristocetin cofactor activity, VWF:GP1bM and VWF propeptide were measured in citrated plasma and platelet-VWF binding in whole blood from 120 healthy individuals to establish a reference interval for active VWF and to assess associations with other VWF parameters. Results Intra- and inter-assay CVs were between 2.4–7.2% and 4.1–9.4%, depending on the level. Mean recovery of spiked recombinant R1306W VWF was 103±3%. The assay was linear in the range of 90.1–424.5% and had a limit of quantification of 101%. The reference interval for active VWF was 91.6–154.8% of NPP. Significant, positive correlations between active VWF and all other VWF parameters were found, with the strongest correlation with VWF:GP1bM binding. Conclusions We developed and validated an immunosorbent assay for the accurate detection of active VWF levels in plasma. The assay fulfilled all analytical criteria in this study and a reference interval was established, allowing its use to quantify active VWF in pathological conditions for future research." @default.
• W2912204111 created "2019-02-21" @default.
• W2912204111 creator A5015701961 @default.
• W2912204111 creator A5017914492 @default.
• W2912204111 creator A5025899708 @default.
• W2912204111 creator A5027231306 @default.
• W2912204111 creator A5027475930 @default.
• W2912204111 creator A5037662846 @default.
• W2912204111 creator A5045390891 @default.
• W2912204111 creator A5064638391 @default.
• W2912204111 creator A5072766555 @default.
• W2912204111 date "2019-02-13" @default.
• W2912204111 modified "2023-10-01" @default.
• W2912204111 title "Analytical characterization and reference interval of an enzyme-linked immunosorbent assay for active von Willebrand factor" @default.
• W2912204111 cites W1895498052 @default.
• W2912204111 cites W1942272692 @default.
• W2912204111 cites W1955380680 @default.
• W2912204111 cites W1965404104 @default.
• W2912204111 cites W1973762835 @default.
• W2912204111 cites W1978557995 @default.
• W2912204111 cites W1982096415 @default.
• W2912204111 cites W1985431824 @default.
• W2912204111 cites W1985726492 @default.
• W2912204111 cites W1997122596 @default.
• W2912204111 cites W1999559827 @default.
• W2912204111 cites W2006378116 @default.
• W2912204111 cites W2008330941 @default.
• W2912204111 cites W2019789419 @default.
• W2912204111 cites W2025396032 @default.
• W2912204111 cites W2029614255 @default.
• W2912204111 cites W2040470931 @default.
• W2912204111 cites W2040553983 @default.
• W2912204111 cites W2041300580 @default.
• W2912204111 cites W2050370340 @default.
• W2912204111 cites W2056849041 @default.
• W2912204111 cites W2057751991 @default.
• W2912204111 cites W2071539899 @default.
• W2912204111 cites W2075013504 @default.
• W2912204111 cites W2085435652 @default.
• W2912204111 cites W2096735552 @default.
• W2912204111 cites W2115956404 @default.
• W2912204111 cites W2122536819 @default.
• W2912204111 cites W2125670243 @default.
• W2912204111 cites W2157575017 @default.
• W2912204111 cites W2329931602 @default.
• W2912204111 cites W2345520253 @default.
• W2912204111 cites W2366110303 @default.
• W2912204111 cites W2408854760 @default.
• W2912204111 cites W2707883 @default.
• W2912204111 cites W2744117793 @default.
• W2912204111 cites W2781181150 @default.
• W2912204111 cites W2792979393 @default.
• W2912204111 cites W2806623628 @default.
• W2912204111 cites W2894771152 @default.
• W2912204111 cites W3113274326 @default.
• W2912204111 doi "https://doi.org/10.1371/journal.pone.0211961" @default.
• W2912204111 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6373957" @default.
• W2912204111 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30759116" @default.
• W2912204111 hasPublicationYear "2019" @default.
• W2912204111 type Work @default.
• W2912204111 sameAs 2912204111 @default.
• W2912204111 citedByCount "16" @default.
• W2912204111 countsByYear W29122041112019 @default.
• W2912204111 countsByYear W29122041112020 @default.
• W2912204111 countsByYear W29122041112021 @default.
• W2912204111 countsByYear W29122041112022 @default.
• W2912204111 countsByYear W29122041112023 @default.
• W2912204111 crossrefType "journal-article" @default.
• W2912204111 hasAuthorship W2912204111A5015701961 @default.
• W2912204111 hasAuthorship W2912204111A5017914492 @default.
• W2912204111 hasAuthorship W2912204111A5025899708 @default.
• W2912204111 hasAuthorship W2912204111A5027231306 @default.
• W2912204111 hasAuthorship W2912204111A5027475930 @default.
• W2912204111 hasAuthorship W2912204111A5037662846 @default.
• W2912204111 hasAuthorship W2912204111A5045390891 @default.
• W2912204111 hasAuthorship W2912204111A5064638391 @default.
• W2912204111 hasAuthorship W2912204111A5072766555 @default.
• W2912204111 hasBestOaLocation W29122041111 @default.
• W2912204111 hasConcept C104317684 @default.
• W2912204111 hasConcept C108625454 @default.
• W2912204111 hasConcept C126322002 @default.
• W2912204111 hasConcept C153911025 @default.
• W2912204111 hasConcept C159654299 @default.
• W2912204111 hasConcept C185592680 @default.
• W2912204111 hasConcept C203014093 @default.
• W2912204111 hasConcept C26828662 @default.
• W2912204111 hasConcept C2776057759 @default.
• W2912204111 hasConcept C2778143274 @default.
• W2912204111 hasConcept C2778442980 @default.
• W2912204111 hasConcept C2778780528 @default.
• W2912204111 hasConcept C2779394231 @default.
• W2912204111 hasConcept C2910597130 @default.
• W2912204111 hasConcept C40767141 @default.
• W2912204111 hasConcept C55493867 @default.
• W2912204111 hasConcept C71924100 @default.
• W2912204111 hasConcept C86803240 @default.
• W2912204111 hasConcept C89560881 @default.
• W2912204111 hasConceptScore W2912204111C104317684 @default.

• next