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• W2912225818 abstract "In highly aggressive malignancies like pancreatic cancer (PC), patient-derived tumor models can serve as disease-relevant models to understand disease-related biology as well as to guide clinical decision-making. In this study, we describe a two-step protocol allowing systematic establishment of patient-derived primary cultures from PC patient tumors. Initial xenotransplantation of surgically resected patient tumors (n = 134) into immunodeficient mice allows for efficient in vivo expansion of vital tumor cells and successful tumor expansion in 38% of patient tumors (51/134). Expansion xenografts closely recapitulate the histoarchitecture of their matching patients’ primary tumors. Digestion of xenograft tumors and subsequent in vitro cultivation resulted in the successful generation of semi-adherent PC cultures of pure epithelial cell origin in 43.1% of the cases. The established primary cultures include diverse pathological types of PC: Pancreatic ductal adenocarcinoma (86.3%, 19/22), adenosquamous carcinoma (9.1%, 2/22) and ductal adenocarcinoma with oncocytic IPMN (4.5%, 1/22). We here provide a protocol to establish quality-controlled PC patient-derived primary cell cultures from heterogeneous PC patient tumors. In vitro preclinical models provide the basis for the identification and preclinical assessment of novel therapeutic opportunities targeting pancreatic cancer." @default.
• W2912225818 created "2019-02-21" @default.
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• W2912225818 date "2019-02-10" @default.
• W2912225818 modified "2023-10-12" @default.
• W2912225818 title "Systematic Generation of Patient-Derived Tumor Models in Pancreatic Cancer" @default.
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• W2912225818 doi "https://doi.org/10.3390/cells8020142" @default.
• W2912225818 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6406729" @default.
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