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• W2912240548 abstract "A competitive fluorescence immunoassay for the identification and quantification of morphine has been developed on the basis of hapten-coated plate format. Hapten was prepared through covalent conjugating a morphine derivative with albumin bovine. In the immunoassay, the hapten was inoculated on a 96-well plate and then bound with monoclonal antibodies labeled with a signal indicating dye, fluorescein isothiocyanate (FITC). Unbound FITC-antibodies were rinsed off from the plate. The fluorescein intensity decreases in the presence of morphine molecules due to the competitively binding to antibodies against hapten. The intensity is inversely correlated with the concentration of morphine. In quantitative analysis for urine samples, we obtained a linearity range of 0.2 μg/mL∼2.5 μg/mL, along with a detection limit of c.a. 1 ng/mL. The fluorescence immunoassay shows low cross-reactivity (below 10%) to 6-acetylmorphine, 3-acetylmorphine, and heroine. The developed method produced comparable results to the standard GC-MS/MS method. In conclusion, a rapid and efficient screening tool for morphine in clinical human urine has been established." @default.
• W2912240548 created "2019-02-21" @default.
• W2912240548 creator A5046127285 @default.
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• W2912240548 date "2019-02-03" @default.
• W2912240548 modified "2023-10-15" @default.
• W2912240548 title "Determination of Morphine in Human Urine by the Novel Competitive Fluorescence Immunoassay" @default.
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• W2912240548 doi "https://doi.org/10.1155/2019/7826090" @default.
• W2912240548 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6377988" @default.
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