Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912240677> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2912240677 endingPage "54a" @default.
- W2912240677 startingPage "54a" @default.
- W2912240677 abstract "The function of integral membrane proteins, such as ion channels, depends not only on the physical properties of the surrounding lipid bilayer but also the identity of lipids, which may interact specifically with the protein, altering functional characteristics of the protein. Here, we investigate the lipid requirement of the bacterial cyclic nucleotide-gated (CNG) channel SthK and how different lipids affect channel function. Density maps from single particle cryoEM structures of SthK reconstituted into lipid nanodiscs revealed lipids located in close proximity to SthK at the protein-membrane interface. In addition, lipid density was also identified along the outside of the inner pore helix, a location less accessible for protein-surrounding lipids. The same lipid densities were observed in SthK structures solved in detergent micelles, indicating that these lipids were co-purified with the channel from E. coli. Thin layer chromatography confirmed the presence of lipids bound to SthK in detergent and, together with mass spectrometry on the same sample, we were able to identify these lipids as POPG and Cardiolipin. We then systematically analyzed the effects of these lipids on channel activity by using a fluorescence-based stopped-flow flux assay. In the absence of negatively charged lipids SthK shows little to no activity indicating that the co-purified lipids are not sufficient to activate the channel. Increasing the amount of POPG or Cardiolipin during the reconstitution leads to significantly increased and stable channel activity. The time course of activation with cAMP and the cAMP concentration needed for half activation were not significantly affected by the type of lipid employed. Our structural data combined with the lipid identification by mass spectrometry and the subsequent functional analyses form the groundwork for future studies on the molecular determinants of protein-lipid interactions in SthK." @default.
- W2912240677 created "2019-02-21" @default.
- W2912240677 creator A5000467515 @default.
- W2912240677 creator A5007134585 @default.
- W2912240677 creator A5012291686 @default.
- W2912240677 creator A5042348341 @default.
- W2912240677 creator A5043438781 @default.
- W2912240677 creator A5087777350 @default.
- W2912240677 date "2019-02-01" @default.
- W2912240677 modified "2023-09-30" @default.
- W2912240677 title "Lipid-Modulation of SthK, a Cyclic Nucleotide-Gated Channel" @default.
- W2912240677 doi "https://doi.org/10.1016/j.bpj.2018.11.338" @default.
- W2912240677 hasPublicationYear "2019" @default.
- W2912240677 type Work @default.
- W2912240677 sameAs 2912240677 @default.
- W2912240677 citedByCount "0" @default.
- W2912240677 crossrefType "journal-article" @default.
- W2912240677 hasAuthorship W2912240677A5000467515 @default.
- W2912240677 hasAuthorship W2912240677A5007134585 @default.
- W2912240677 hasAuthorship W2912240677A5012291686 @default.
- W2912240677 hasAuthorship W2912240677A5042348341 @default.
- W2912240677 hasAuthorship W2912240677A5043438781 @default.
- W2912240677 hasAuthorship W2912240677A5087777350 @default.
- W2912240677 hasBestOaLocation W29122406771 @default.
- W2912240677 hasConcept C104317684 @default.
- W2912240677 hasConcept C121332964 @default.
- W2912240677 hasConcept C123079801 @default.
- W2912240677 hasConcept C12554922 @default.
- W2912240677 hasConcept C127162648 @default.
- W2912240677 hasConcept C185592680 @default.
- W2912240677 hasConcept C24890656 @default.
- W2912240677 hasConcept C2778524494 @default.
- W2912240677 hasConcept C41008148 @default.
- W2912240677 hasConcept C512185932 @default.
- W2912240677 hasConcept C55493867 @default.
- W2912240677 hasConcept C76155785 @default.
- W2912240677 hasConcept C86803240 @default.
- W2912240677 hasConceptScore W2912240677C104317684 @default.
- W2912240677 hasConceptScore W2912240677C121332964 @default.
- W2912240677 hasConceptScore W2912240677C123079801 @default.
- W2912240677 hasConceptScore W2912240677C12554922 @default.
- W2912240677 hasConceptScore W2912240677C127162648 @default.
- W2912240677 hasConceptScore W2912240677C185592680 @default.
- W2912240677 hasConceptScore W2912240677C24890656 @default.
- W2912240677 hasConceptScore W2912240677C2778524494 @default.
- W2912240677 hasConceptScore W2912240677C41008148 @default.
- W2912240677 hasConceptScore W2912240677C512185932 @default.
- W2912240677 hasConceptScore W2912240677C55493867 @default.
- W2912240677 hasConceptScore W2912240677C76155785 @default.
- W2912240677 hasConceptScore W2912240677C86803240 @default.
- W2912240677 hasIssue "3" @default.
- W2912240677 hasLocation W29122406771 @default.
- W2912240677 hasOpenAccess W2912240677 @default.
- W2912240677 hasPrimaryLocation W29122406771 @default.
- W2912240677 hasRelatedWork W1201332208 @default.
- W2912240677 hasRelatedWork W1512280543 @default.
- W2912240677 hasRelatedWork W2083202312 @default.
- W2912240677 hasRelatedWork W2088033892 @default.
- W2912240677 hasRelatedWork W2126308538 @default.
- W2912240677 hasRelatedWork W2184915363 @default.
- W2912240677 hasRelatedWork W2757001561 @default.
- W2912240677 hasRelatedWork W2809452511 @default.
- W2912240677 hasRelatedWork W2887343856 @default.
- W2912240677 hasRelatedWork W2909969839 @default.
- W2912240677 hasRelatedWork W2912873065 @default.
- W2912240677 hasRelatedWork W2915062120 @default.
- W2912240677 hasRelatedWork W2976166900 @default.
- W2912240677 hasRelatedWork W2976651276 @default.
- W2912240677 hasRelatedWork W3020927568 @default.
- W2912240677 hasRelatedWork W3029876403 @default.
- W2912240677 hasRelatedWork W3030191893 @default.
- W2912240677 hasRelatedWork W762686358 @default.
- W2912240677 hasRelatedWork W2751141108 @default.
- W2912240677 hasRelatedWork W3105342812 @default.
- W2912240677 hasVolume "116" @default.
- W2912240677 isParatext "false" @default.
- W2912240677 isRetracted "false" @default.
- W2912240677 magId "2912240677" @default.
- W2912240677 workType "article" @default.