FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912253942> ?p ?o ?g. }

• W2912253942 abstract "Bear bile powder is a valuable medicinal material characterized by high content of tauroursodeoxycholic acid (TUDCA) at a certain ratio to taurochenodeoxycholic acid (TCDCA). We had created an engineered E. coli harboring two-step bidirectional oxidative and reductive enzyme-catalyzing pathway that could rapidly convert TCDCA to TUDCA at a specific percentage in shake flasks. We reported here the large-scale production of TUDCA containing products by balancing the bidirectional reactions through optimizing fermentation process of the engineered E. coli in fermenters. The fermentation medium was firstly optimized based on M9 medium using response surface methodology, leading to a glycerol and yeast extract modified M9-GY medium benefits for both cell growth and product conversion efficiency. Then isopropylthio-β-galactoside induction and fed-stock stage was successively optimized. Finally, a special deep-tank static process was developed to promote the conversion from TCDCA to TUDCA. Applying the optimal condition, fermentation was performed by separately supplementing 30 g refined chicken bile powder and 35 g crude chicken bile powder as substrates, resulting in 29.35 ± 2.83 g and 30.78 ± 3.04 g powder products containing 35.85 ± 3.85% and 27.14 ± 4.23% of TUDCA at a ratio of 1.49 ± 0.14 and 1.55 ± 0.19 to TCDCA, respectively, after purification and evaporation of the fermentation broth. The recovery yield was 92.84 ± 4.21% and 91.83 ± 2.56%, respectively. This study provided a practical and environment friendly industrialized process for producing artificial substitute of bear bile powder from cheap and readily available chicken bile powder using engineered E. coli microbial cell factory. It also put forward an interesting deep-tank static process to promote the enzyme-catalyzing reactions toward target compounds in synthetic biology-based fermentation." @default.
• W2912253942 created "2019-02-21" @default.
• W2912253942 creator A5000891681 @default.
• W2912253942 creator A5041541653 @default.
• W2912253942 creator A5050502206 @default.
• W2912253942 creator A5065468740 @default.
• W2912253942 date "2019-02-08" @default.
• W2912253942 modified "2023-10-18" @default.
• W2912253942 title "Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory" @default.
• W2912253942 cites W1484118677 @default.
• W2912253942 cites W1977677401 @default.
• W2912253942 cites W1988333667 @default.
• W2912253942 cites W1990062646 @default.
• W2912253942 cites W1993062864 @default.
• W2912253942 cites W1998240064 @default.
• W2912253942 cites W1998701868 @default.
• W2912253942 cites W2004161015 @default.
• W2912253942 cites W2015215288 @default.
• W2912253942 cites W2022477950 @default.
• W2912253942 cites W2024172386 @default.
• W2912253942 cites W2035455980 @default.
• W2912253942 cites W2038218477 @default.
• W2912253942 cites W2044276126 @default.
• W2912253942 cites W2050225360 @default.
• W2912253942 cites W2064122930 @default.
• W2912253942 cites W2065051999 @default.
• W2912253942 cites W2066607937 @default.
• W2912253942 cites W2068369979 @default.
• W2912253942 cites W2070278261 @default.
• W2912253942 cites W2072539309 @default.
• W2912253942 cites W2076566455 @default.
• W2912253942 cites W2078287418 @default.
• W2912253942 cites W2092528358 @default.
• W2912253942 cites W2096634254 @default.
• W2912253942 cites W2100324626 @default.
• W2912253942 cites W2102957972 @default.
• W2912253942 cites W2135303614 @default.
• W2912253942 cites W2142082954 @default.
• W2912253942 cites W2145922781 @default.
• W2912253942 cites W2147516046 @default.
• W2912253942 cites W2159314121 @default.
• W2912253942 cites W2268117688 @default.
• W2912253942 cites W2302749214 @default.
• W2912253942 cites W2435600814 @default.
• W2912253942 cites W2470133246 @default.
• W2912253942 cites W2528149983 @default.
• W2912253942 cites W2552042822 @default.
• W2912253942 cites W2599157787 @default.
• W2912253942 cites W2621032226 @default.
• W2912253942 cites W2767723018 @default.
• W2912253942 cites W2806937873 @default.
• W2912253942 cites W2883453302 @default.
• W2912253942 cites W3003977897 @default.
• W2912253942 doi "https://doi.org/10.1186/s12934-019-1076-2" @default.
• W2912253942 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6368744" @default.
• W2912253942 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30736766" @default.
• W2912253942 hasPublicationYear "2019" @default.
• W2912253942 type Work @default.
• W2912253942 sameAs 2912253942 @default.
• W2912253942 citedByCount "9" @default.
• W2912253942 countsByYear W29122539422019 @default.
• W2912253942 countsByYear W29122539422021 @default.
• W2912253942 countsByYear W29122539422022 @default.
• W2912253942 countsByYear W29122539422023 @default.
• W2912253942 crossrefType "journal-article" @default.
• W2912253942 hasAuthorship W2912253942A5000891681 @default.
• W2912253942 hasAuthorship W2912253942A5041541653 @default.
• W2912253942 hasAuthorship W2912253942A5050502206 @default.
• W2912253942 hasAuthorship W2912253942A5065468740 @default.
• W2912253942 hasBestOaLocation W29122539421 @default.
• W2912253942 hasConcept C100544194 @default.
• W2912253942 hasConcept C104317684 @default.
• W2912253942 hasConcept C127413603 @default.
• W2912253942 hasConcept C139447449 @default.
• W2912253942 hasConcept C158617107 @default.
• W2912253942 hasConcept C185592680 @default.
• W2912253942 hasConcept C2777422422 @default.
• W2912253942 hasConcept C31903555 @default.
• W2912253942 hasConcept C528095902 @default.
• W2912253942 hasConcept C547475151 @default.
• W2912253942 hasConcept C55493867 @default.
• W2912253942 hasConcept C73401843 @default.
• W2912253942 hasConceptScore W2912253942C100544194 @default.
• W2912253942 hasConceptScore W2912253942C104317684 @default.
• W2912253942 hasConceptScore W2912253942C127413603 @default.
• W2912253942 hasConceptScore W2912253942C139447449 @default.
• W2912253942 hasConceptScore W2912253942C158617107 @default.
• W2912253942 hasConceptScore W2912253942C185592680 @default.
• W2912253942 hasConceptScore W2912253942C2777422422 @default.
• W2912253942 hasConceptScore W2912253942C31903555 @default.
• W2912253942 hasConceptScore W2912253942C528095902 @default.
• W2912253942 hasConceptScore W2912253942C547475151 @default.
• W2912253942 hasConceptScore W2912253942C55493867 @default.
• W2912253942 hasConceptScore W2912253942C73401843 @default.
• W2912253942 hasFunder F4320321001 @default.
• W2912253942 hasIssue "1" @default.
• W2912253942 hasLocation W29122539421 @default.
• W2912253942 hasLocation W29122539422 @default.
• W2912253942 hasLocation W29122539423 @default.
• W2912253942 hasLocation W29122539424 @default.

• next