FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912256178> ?p ?o ?g. }

• W2912256178 endingPage "628" @default.
• W2912256178 startingPage "613" @default.
• W2912256178 abstract "Abstract Proteins are dynamic in nature and exist in a set of equilibrium conformations on various timescale motions. The flexibility of proteins governs various biological functions, and therefore elucidation of such functional dynamics is essential. In this context, we have studied the structure–dynamics–stability–activity relationship of bacteriophage T7 lysozyme/endolysin (T7L) native-state ensemble in the pH range of 6–8. Our studies established that T7L native state is conformationally heterogeneous, as several residues of its C-terminal half are present in two conformations (major and minor) in the slow exchange time scale of nuclear magnetic resonance (NMR). Structural and dynamic studies suggested that the residues belonging to minor conformations do exhibit native-like structural and dynamic features. Furthermore, the NMR relaxation experiments unraveled that the native state is highly dynamic and the dynamic behavior is regulated by the pH, as the pH 6 conformation exhibited enhanced dynamics compared with pH 7 and 8. The stability measurements and cell-based activity studies on T7L indicated that the native protein at pH 6 is ∼2 kcal less stable and is ∼50% less active than those of pH 7 and 8. A comprehensive analysis of the T7L active site, unfolding initiation sites and the residues with altered dynamics outlined that the attenuation of stability and activity is a resultant of its enhanced dynamic properties, which, in turn, can be attributed to the protonation/deprotonation of its partially buried His residues. Our study on T7L structure–dynamics–activity paradigm could assist in engineering novel amidase-based endolysins with enhanced activity and stability over a broad pH range." @default.
• W2912256178 created "2019-02-21" @default.
• W2912256178 creator A5023454945 @default.
• W2912256178 creator A5037894792 @default.
• W2912256178 creator A5040054702 @default.
• W2912256178 creator A5055223793 @default.
• W2912256178 date "2019-02-14" @default.
• W2912256178 modified "2023-10-16" @default.
• W2912256178 title "Enhanced dynamics of conformationally heterogeneous T7 bacteriophage lysozyme native state attenuates its stability and activity" @default.
• W2912256178 cites W1472491453 @default.
• W2912256178 cites W1513002528 @default.
• W2912256178 cites W1600771742 @default.
• W2912256178 cites W1601592303 @default.
• W2912256178 cites W1864887623 @default.
• W2912256178 cites W1877414581 @default.
• W2912256178 cites W1965076207 @default.
• W2912256178 cites W1966533809 @default.
• W2912256178 cites W1979671672 @default.
• W2912256178 cites W1981399542 @default.
• W2912256178 cites W1985767604 @default.
• W2912256178 cites W1987592314 @default.
• W2912256178 cites W198782672 @default.
• W2912256178 cites W1994009390 @default.
• W2912256178 cites W1994632567 @default.
• W2912256178 cites W1996859443 @default.
• W2912256178 cites W1999883559 @default.
• W2912256178 cites W2002841189 @default.
• W2912256178 cites W2013082923 @default.
• W2912256178 cites W2015117663 @default.
• W2912256178 cites W2016654903 @default.
• W2912256178 cites W2017492486 @default.
• W2912256178 cites W2019147544 @default.
• W2912256178 cites W2025088751 @default.
• W2912256178 cites W2034601153 @default.
• W2912256178 cites W2036996222 @default.
• W2912256178 cites W2037021218 @default.
• W2912256178 cites W2037706600 @default.
• W2912256178 cites W2041735927 @default.
• W2912256178 cites W2045950270 @default.
• W2912256178 cites W2048822589 @default.
• W2912256178 cites W2050426766 @default.
• W2912256178 cites W2059145665 @default.
• W2912256178 cites W2060002424 @default.
• W2912256178 cites W2060620216 @default.
• W2912256178 cites W2061060115 @default.
• W2912256178 cites W2063654429 @default.
• W2912256178 cites W2076911982 @default.
• W2912256178 cites W2087432921 @default.
• W2912256178 cites W2091765279 @default.
• W2912256178 cites W2091863103 @default.
• W2912256178 cites W2093969953 @default.
• W2912256178 cites W2094795801 @default.
• W2912256178 cites W2102034855 @default.
• W2912256178 cites W2113926549 @default.
• W2912256178 cites W2129879061 @default.
• W2912256178 cites W2133218818 @default.
• W2912256178 cites W2133762037 @default.
• W2912256178 cites W2140926876 @default.
• W2912256178 cites W2141218040 @default.
• W2912256178 cites W2147106126 @default.
• W2912256178 cites W2147466870 @default.
• W2912256178 cites W2148521301 @default.
• W2912256178 cites W2157255054 @default.
• W2912256178 cites W2165186407 @default.
• W2912256178 cites W2214655780 @default.
• W2912256178 cites W2288318116 @default.
• W2912256178 cites W2320112178 @default.
• W2912256178 cites W2325252308 @default.
• W2912256178 cites W2417252461 @default.
• W2912256178 cites W2513276674 @default.
• W2912256178 cites W2525524736 @default.
• W2912256178 cites W2754807338 @default.
• W2912256178 cites W2784088838 @default.
• W2912256178 cites W2951648237 @default.
• W2912256178 cites W4019147 @default.
• W2912256178 doi "https://doi.org/10.1042/bcj20180703" @default.
• W2912256178 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30683715" @default.
• W2912256178 hasPublicationYear "2019" @default.
• W2912256178 type Work @default.
• W2912256178 sameAs 2912256178 @default.
• W2912256178 citedByCount "12" @default.
• W2912256178 countsByYear W29122561782019 @default.
• W2912256178 countsByYear W29122561782020 @default.
• W2912256178 countsByYear W29122561782021 @default.
• W2912256178 countsByYear W29122561782022 @default.
• W2912256178 countsByYear W29122561782023 @default.
• W2912256178 crossrefType "journal-article" @default.
• W2912256178 hasAuthorship W2912256178A5023454945 @default.
• W2912256178 hasAuthorship W2912256178A5037894792 @default.
• W2912256178 hasAuthorship W2912256178A5040054702 @default.
• W2912256178 hasAuthorship W2912256178A5055223793 @default.
• W2912256178 hasConcept C12554922 @default.
• W2912256178 hasConcept C145148216 @default.
• W2912256178 hasConcept C147597530 @default.
• W2912256178 hasConcept C151730666 @default.
• W2912256178 hasConcept C178790620 @default.
• W2912256178 hasConcept C181199279 @default.
• W2912256178 hasConcept C185592680 @default.

• next