Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912256384> ?p ?o ?g. }
- W2912256384 endingPage "283" @default.
- W2912256384 startingPage "272" @default.
- W2912256384 abstract "A multiprotein complex, THO/TREX, couples the transcription, 3'-end formation and nuclear export of mRNAs. In this study, we report that crucial factors for mRNA processing, such as XRN2, DDX5/DDX17 and CstF64, are copurified with human THO (hTHO). Using chromatin immunoprecipitation, we found increased cross-linking of XRN2 and CstF64 to the RNA polymerase II (RNAP II) pause site of the HSPA1A gene upon down-regulation of THOC5, a metazoan-specific component of hTHO. As observed in THOC5-depleted cells, knockdown of XRN2 blocked HSP70 transcript release and increased the amount of CstF64 at the pause site. In addition, our data indicate that DDX5/DDX17 is also required for HSP70 transcript release. As the degradation of read-through transcripts, but not cleavage at polyadenylation sites per se, was hindered upon THOC5 or DDX5/DDX17 down-regulation, these factors appear to influence transcriptional termination. Interestingly, over-expression of RNase H suppressed the accumulation of HSP70 transcripts in nuclear foci in THOC5- or DDX5/DDX17-depleted cells. Thus, we propose a model in which hTHO, along with DDX5/DDX17, restricts the formation of R-loops, thereby facilitating the XRN2-mediated transcriptional termination and release of the mature transcript from the HSPA1A locus." @default.
- W2912256384 created "2019-02-21" @default.
- W2912256384 creator A5026640503 @default.
- W2912256384 creator A5032840258 @default.
- W2912256384 creator A5056305013 @default.
- W2912256384 creator A5079343366 @default.
- W2912256384 creator A5088207071 @default.
- W2912256384 date "2019-02-27" @default.
- W2912256384 modified "2023-10-16" @default.
- W2912256384 title "Human THO coordinates transcription termination and subsequent transcript release from the <i>HSP70</i> locus" @default.
- W2912256384 cites W1909699921 @default.
- W2912256384 cites W1963504189 @default.
- W2912256384 cites W1963848932 @default.
- W2912256384 cites W1966735041 @default.
- W2912256384 cites W1969131246 @default.
- W2912256384 cites W1974434328 @default.
- W2912256384 cites W1977783165 @default.
- W2912256384 cites W1981232162 @default.
- W2912256384 cites W1989967763 @default.
- W2912256384 cites W1993620549 @default.
- W2912256384 cites W1995842607 @default.
- W2912256384 cites W2000702411 @default.
- W2912256384 cites W2002881421 @default.
- W2912256384 cites W2003098836 @default.
- W2912256384 cites W2004731223 @default.
- W2912256384 cites W2007708215 @default.
- W2912256384 cites W2011726525 @default.
- W2912256384 cites W2016867961 @default.
- W2912256384 cites W2023771912 @default.
- W2912256384 cites W2028061670 @default.
- W2912256384 cites W2038211069 @default.
- W2912256384 cites W2038424676 @default.
- W2912256384 cites W2042975193 @default.
- W2912256384 cites W2043664938 @default.
- W2912256384 cites W2051465752 @default.
- W2912256384 cites W2058654089 @default.
- W2912256384 cites W2067877852 @default.
- W2912256384 cites W2074577885 @default.
- W2912256384 cites W2076091831 @default.
- W2912256384 cites W2080596357 @default.
- W2912256384 cites W2093900943 @default.
- W2912256384 cites W2095332284 @default.
- W2912256384 cites W2100041522 @default.
- W2912256384 cites W2100959401 @default.
- W2912256384 cites W2108102052 @default.
- W2912256384 cites W2115521704 @default.
- W2912256384 cites W2116635614 @default.
- W2912256384 cites W2116764585 @default.
- W2912256384 cites W2118929024 @default.
- W2912256384 cites W2128422010 @default.
- W2912256384 cites W2129214044 @default.
- W2912256384 cites W2131824470 @default.
- W2912256384 cites W2136938925 @default.
- W2912256384 cites W2140970597 @default.
- W2912256384 cites W2148624148 @default.
- W2912256384 cites W2155077792 @default.
- W2912256384 cites W2157253966 @default.
- W2912256384 cites W2160720092 @default.
- W2912256384 cites W2161887614 @default.
- W2912256384 cites W2163180503 @default.
- W2912256384 cites W2167078003 @default.
- W2912256384 cites W2170808955 @default.
- W2912256384 cites W2172276712 @default.
- W2912256384 cites W2225270156 @default.
- W2912256384 cites W2264951480 @default.
- W2912256384 cites W2340421620 @default.
- W2912256384 cites W2399619985 @default.
- W2912256384 cites W2416819443 @default.
- W2912256384 cites W2474586925 @default.
- W2912256384 cites W2503054853 @default.
- W2912256384 cites W2595837675 @default.
- W2912256384 cites W2736275121 @default.
- W2912256384 cites W2785666349 @default.
- W2912256384 cites W2800358055 @default.
- W2912256384 cites W2884601122 @default.
- W2912256384 cites W2889815145 @default.
- W2912256384 doi "https://doi.org/10.1111/gtc.12672" @default.
- W2912256384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30721563" @default.
- W2912256384 hasPublicationYear "2019" @default.
- W2912256384 type Work @default.
- W2912256384 sameAs 2912256384 @default.
- W2912256384 citedByCount "6" @default.
- W2912256384 countsByYear W29122563842020 @default.
- W2912256384 countsByYear W29122563842021 @default.
- W2912256384 countsByYear W29122563842022 @default.
- W2912256384 countsByYear W29122563842023 @default.
- W2912256384 crossrefType "journal-article" @default.
- W2912256384 hasAuthorship W2912256384A5026640503 @default.
- W2912256384 hasAuthorship W2912256384A5032840258 @default.
- W2912256384 hasAuthorship W2912256384A5056305013 @default.
- W2912256384 hasAuthorship W2912256384A5079343366 @default.
- W2912256384 hasAuthorship W2912256384A5088207071 @default.
- W2912256384 hasBestOaLocation W29122563841 @default.
- W2912256384 hasConcept C101762097 @default.
- W2912256384 hasConcept C104317684 @default.
- W2912256384 hasConcept C105580179 @default.
- W2912256384 hasConcept C134320426 @default.
- W2912256384 hasConcept C138885662 @default.