Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912265569> ?p ?o ?g. }
- W2912265569 endingPage "708" @default.
- W2912265569 startingPage "708" @default.
- W2912265569 abstract "Fisetin, a dietary flavonoid, is reported to have cellular antioxidant activity with an unclear mechanism. In this study, we investigated the effect of fisetin on the nuclear factor, erythroid 2-like 2 (Nrf2) signaling pathway in HepG2 cells to explore the cellular antioxidant mechanism. Fisetin upregulated the mRNA expression of heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), and NAD(P)H quinone oxidoreductase-1 (NQO1), and induced the protein of HO-1 but had no significant effect on the protein of GCLC, GCLM and NQO1. Moreover, nuclear accumulation of Nrf2 was clearly observed by immunofluorescence analysis and western blotting after fisetin treatment, and an enhanced luciferase activity of antioxidant response element (ARE)-regulated transactivation was obtained by dual-luciferase reporter gene assays. In addition, fisetin upregulated the protein level of Nrf2 and downregulated the protein level of Kelch-like ECH-associated protein 1 (Keap1). However, fisetin had no significant effect on Nrf2 mRNA expression. When protein synthesis was inhibited with cycloheximide (CHX), fisetin prolonged the half-life of Nrf2 from 15 min to 45 min. When blocking Nrf2 degradation with proteasome inhibitor MG132, ubiquitinated proteins were enhanced, and fisetin reduced ubiquitination of Nrf2. Taken together, fisetin translocated Nrf2 into the nucleus and upregulated the expression of downstream HO-1 gene by inhibiting the degradation of Nrf2 at the post-transcriptional level. These data provide the molecular mechanism to understand the cellular antioxidant activity of fisetin." @default.
- W2912265569 created "2019-02-21" @default.
- W2912265569 creator A5023369477 @default.
- W2912265569 creator A5025633795 @default.
- W2912265569 creator A5031733543 @default.
- W2912265569 creator A5038994914 @default.
- W2912265569 creator A5049917503 @default.
- W2912265569 creator A5068194864 @default.
- W2912265569 creator A5071832127 @default.
- W2912265569 creator A5089024357 @default.
- W2912265569 date "2019-02-15" @default.
- W2912265569 modified "2023-10-06" @default.
- W2912265569 title "Nrf2–ARE Signaling Acts as Master Pathway for the Cellular Antioxidant Activity of Fisetin" @default.
- W2912265569 cites W1227142308 @default.
- W2912265569 cites W1659912652 @default.
- W2912265569 cites W1732537321 @default.
- W2912265569 cites W1802944057 @default.
- W2912265569 cites W1989373139 @default.
- W2912265569 cites W2007686006 @default.
- W2912265569 cites W2008644600 @default.
- W2912265569 cites W2015392958 @default.
- W2912265569 cites W2017498511 @default.
- W2912265569 cites W2018457932 @default.
- W2912265569 cites W2024436789 @default.
- W2912265569 cites W2025290835 @default.
- W2912265569 cites W2031588686 @default.
- W2912265569 cites W2045538216 @default.
- W2912265569 cites W2054733228 @default.
- W2912265569 cites W2059447116 @default.
- W2912265569 cites W2077408869 @default.
- W2912265569 cites W2080435498 @default.
- W2912265569 cites W2084038129 @default.
- W2912265569 cites W2094156327 @default.
- W2912265569 cites W2117741334 @default.
- W2912265569 cites W2134974848 @default.
- W2912265569 cites W2150569822 @default.
- W2912265569 cites W2153112084 @default.
- W2912265569 cites W2155324869 @default.
- W2912265569 cites W2166329058 @default.
- W2912265569 cites W2235085556 @default.
- W2912265569 cites W2276027104 @default.
- W2912265569 cites W2286748995 @default.
- W2912265569 cites W2312989214 @default.
- W2912265569 cites W2327579767 @default.
- W2912265569 cites W2341642068 @default.
- W2912265569 cites W2404878622 @default.
- W2912265569 cites W2417468040 @default.
- W2912265569 cites W2606436918 @default.
- W2912265569 cites W2620241301 @default.
- W2912265569 cites W2755558750 @default.
- W2912265569 cites W2756811299 @default.
- W2912265569 cites W2766388141 @default.
- W2912265569 cites W2768455179 @default.
- W2912265569 cites W2769622412 @default.
- W2912265569 cites W2804128670 @default.
- W2912265569 cites W2891794739 @default.
- W2912265569 cites W2908925188 @default.
- W2912265569 cites W904590016 @default.
- W2912265569 doi "https://doi.org/10.3390/molecules24040708" @default.
- W2912265569 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6413105" @default.
- W2912265569 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30781396" @default.
- W2912265569 hasPublicationYear "2019" @default.
- W2912265569 type Work @default.
- W2912265569 sameAs 2912265569 @default.
- W2912265569 citedByCount "31" @default.
- W2912265569 countsByYear W29122655692019 @default.
- W2912265569 countsByYear W29122655692020 @default.
- W2912265569 countsByYear W29122655692021 @default.
- W2912265569 countsByYear W29122655692022 @default.
- W2912265569 countsByYear W29122655692023 @default.
- W2912265569 crossrefType "journal-article" @default.
- W2912265569 hasAuthorship W2912265569A5023369477 @default.
- W2912265569 hasAuthorship W2912265569A5025633795 @default.
- W2912265569 hasAuthorship W2912265569A5031733543 @default.
- W2912265569 hasAuthorship W2912265569A5038994914 @default.
- W2912265569 hasAuthorship W2912265569A5049917503 @default.
- W2912265569 hasAuthorship W2912265569A5068194864 @default.
- W2912265569 hasAuthorship W2912265569A5071832127 @default.
- W2912265569 hasAuthorship W2912265569A5089024357 @default.
- W2912265569 hasBestOaLocation W29122655691 @default.
- W2912265569 hasConcept C104317684 @default.
- W2912265569 hasConcept C127561419 @default.
- W2912265569 hasConcept C1292079 @default.
- W2912265569 hasConcept C134459356 @default.
- W2912265569 hasConcept C150194340 @default.
- W2912265569 hasConcept C153911025 @default.
- W2912265569 hasConcept C185592680 @default.
- W2912265569 hasConcept C25602115 @default.
- W2912265569 hasConcept C27740335 @default.
- W2912265569 hasConcept C2776529522 @default.
- W2912265569 hasConcept C2777376040 @default.
- W2912265569 hasConcept C2778004101 @default.
- W2912265569 hasConcept C2778367456 @default.
- W2912265569 hasConcept C2778506107 @default.
- W2912265569 hasConcept C2779054382 @default.
- W2912265569 hasConcept C2779881523 @default.
- W2912265569 hasConcept C2781173740 @default.
- W2912265569 hasConcept C55493867 @default.