Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912295420> ?p ?o ?g. }
- W2912295420 endingPage "301" @default.
- W2912295420 startingPage "292" @default.
- W2912295420 abstract "Abstract Norverapamil, the N‐demethylated derivative of verapamil, is a novel promising leading compound for attenuating multidrug resistance with less side effects compared with verapamil. However, the efficient synthetic method for norverapamil is absent. In this study, an innovative biotechnological method based on enzymatic catalysis was presented for the high‐efficient production of norverapamil. CYP105D1, a cytochrome P450 from Streptomyces griseus ATCC 13273, was identified to carry out a one‐step specific N‐demethylation of verapamil along with putidaredoxin reductase (Pdr) and putidaredoxin (Pdx) as the redox partner. Docking calculations rationalized the specific N‐demethylation observed in experiment and identified important amino acid residues for verapamil binding. Furthermore, a CYP105D1‐based whole‐cell system in E. coli BL21(DE3) was established and optimized for highly efficient N‐demethylation of verapamil. The bioconversion rate of verapamil by the whole cell system came up to 60.16% within 24 hours under the optimized conditions. These results demonstrated the high potential of CYP105D1‐based biocatalytic system for norverapamil production." @default.
- W2912295420 created "2019-02-21" @default.
- W2912295420 creator A5001910656 @default.
- W2912295420 creator A5003211711 @default.
- W2912295420 creator A5015041062 @default.
- W2912295420 creator A5020248771 @default.
- W2912295420 creator A5066696317 @default.
- W2912295420 creator A5091176232 @default.
- W2912295420 date "2019-02-25" @default.
- W2912295420 modified "2023-10-17" @default.
- W2912295420 title "Specific N‐demethylation of verapamil by cytochrome P450 from<i>Streptomyces griseus</i>ATCC 13273" @default.
- W2912295420 cites W1585085658 @default.
- W2912295420 cites W1759188384 @default.
- W2912295420 cites W1951469402 @default.
- W2912295420 cites W1971019767 @default.
- W2912295420 cites W1990324427 @default.
- W2912295420 cites W2017581392 @default.
- W2912295420 cites W2023189207 @default.
- W2912295420 cites W2074411241 @default.
- W2912295420 cites W2089719184 @default.
- W2912295420 cites W2113827726 @default.
- W2912295420 cites W2133549463 @default.
- W2912295420 cites W2138791276 @default.
- W2912295420 cites W2139104845 @default.
- W2912295420 cites W2151558718 @default.
- W2912295420 cites W2152368724 @default.
- W2912295420 cites W2163089754 @default.
- W2912295420 cites W2277869106 @default.
- W2912295420 cites W2640195836 @default.
- W2912295420 cites W2765173544 @default.
- W2912295420 cites W2792496404 @default.
- W2912295420 cites W2898198715 @default.
- W2912295420 cites W2951941889 @default.
- W2912295420 doi "https://doi.org/10.1002/elsc.201800116" @default.
- W2912295420 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6999465" @default.
- W2912295420 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32625009" @default.
- W2912295420 hasPublicationYear "2019" @default.
- W2912295420 type Work @default.
- W2912295420 sameAs 2912295420 @default.
- W2912295420 citedByCount "2" @default.
- W2912295420 countsByYear W29122954202022 @default.
- W2912295420 crossrefType "journal-article" @default.
- W2912295420 hasAuthorship W2912295420A5001910656 @default.
- W2912295420 hasAuthorship W2912295420A5003211711 @default.
- W2912295420 hasAuthorship W2912295420A5015041062 @default.
- W2912295420 hasAuthorship W2912295420A5020248771 @default.
- W2912295420 hasAuthorship W2912295420A5066696317 @default.
- W2912295420 hasAuthorship W2912295420A5091176232 @default.
- W2912295420 hasBestOaLocation W29122954201 @default.
- W2912295420 hasConcept C104317684 @default.
- W2912295420 hasConcept C150194340 @default.
- W2912295420 hasConcept C159110408 @default.
- W2912295420 hasConcept C178790620 @default.
- W2912295420 hasConcept C181199279 @default.
- W2912295420 hasConcept C185592680 @default.
- W2912295420 hasConcept C190727270 @default.
- W2912295420 hasConcept C202199734 @default.
- W2912295420 hasConcept C2777022698 @default.
- W2912295420 hasConcept C2779920670 @default.
- W2912295420 hasConcept C2780768313 @default.
- W2912295420 hasConcept C2780873923 @default.
- W2912295420 hasConcept C41685203 @default.
- W2912295420 hasConcept C519063684 @default.
- W2912295420 hasConcept C523546767 @default.
- W2912295420 hasConcept C526171541 @default.
- W2912295420 hasConcept C54355233 @default.
- W2912295420 hasConcept C55493867 @default.
- W2912295420 hasConcept C71240020 @default.
- W2912295420 hasConcept C71924100 @default.
- W2912295420 hasConcept C86803240 @default.
- W2912295420 hasConcept C87644729 @default.
- W2912295420 hasConceptScore W2912295420C104317684 @default.
- W2912295420 hasConceptScore W2912295420C150194340 @default.
- W2912295420 hasConceptScore W2912295420C159110408 @default.
- W2912295420 hasConceptScore W2912295420C178790620 @default.
- W2912295420 hasConceptScore W2912295420C181199279 @default.
- W2912295420 hasConceptScore W2912295420C185592680 @default.
- W2912295420 hasConceptScore W2912295420C190727270 @default.
- W2912295420 hasConceptScore W2912295420C202199734 @default.
- W2912295420 hasConceptScore W2912295420C2777022698 @default.
- W2912295420 hasConceptScore W2912295420C2779920670 @default.
- W2912295420 hasConceptScore W2912295420C2780768313 @default.
- W2912295420 hasConceptScore W2912295420C2780873923 @default.
- W2912295420 hasConceptScore W2912295420C41685203 @default.
- W2912295420 hasConceptScore W2912295420C519063684 @default.
- W2912295420 hasConceptScore W2912295420C523546767 @default.
- W2912295420 hasConceptScore W2912295420C526171541 @default.
- W2912295420 hasConceptScore W2912295420C54355233 @default.
- W2912295420 hasConceptScore W2912295420C55493867 @default.
- W2912295420 hasConceptScore W2912295420C71240020 @default.
- W2912295420 hasConceptScore W2912295420C71924100 @default.
- W2912295420 hasConceptScore W2912295420C86803240 @default.
- W2912295420 hasConceptScore W2912295420C87644729 @default.
- W2912295420 hasFunder F4320327518 @default.
- W2912295420 hasIssue "4" @default.
- W2912295420 hasLocation W29122954201 @default.
- W2912295420 hasLocation W29122954202 @default.
- W2912295420 hasLocation W29122954203 @default.