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• W2912296833 abstract "Abstract In situ measurements of transcription factor (TF) binding are confounded by cellular heterogeneity and represent averaged profiles in complex tissues. Single cell RNA-seq (scRNA-seq) is capable of resolving different cell types based on gene expression profiles, but no technology exists to directly link specific cell types to the binding pattern of TFs in those cell types. Here, we present self-reporting transposons (SRTs) and their use in single cell calling cards (scCC), a novel assay for simultaneously capturing gene expression profiles and mapping TF binding sites in single cells. First, we show how the genomic locations of SRTs can be recovered from mRNA. Next, we demonstrate that SRTs deposited by the piggyBac transposase can be used to map the genome-wide localization of the TFs SP1, through a direct fusion of the two proteins, and BRD4, through its native affinity for piggyBac . We then present the scCC method, which maps SRTs from scRNA-seq libraries, thus enabling concomitant identification of cell types and TF binding sites in those same cells. As a proof-of-concept, we show recovery of cell type-specific BRD4 and SP1 binding sites from cultured cells. Finally, we map Brd4 binding sites in the mouse cortex at single cell resolution, thus establishing a new technique for studying TF biology in situ." @default.
• W2912296833 created "2019-02-21" @default.
• W2912296833 creator A5036601296 @default.
• W2912296833 creator A5041943054 @default.
• W2912296833 creator A5044795455 @default.
• W2912296833 creator A5050964355 @default.
• W2912296833 creator A5060720882 @default.
• W2912296833 creator A5066263942 @default.
• W2912296833 creator A5078607646 @default.
• W2912296833 creator A5084612869 @default.
• W2912296833 creator A5087353754 @default.
• W2912296833 creator A5087903696 @default.
• W2912296833 date "2019-02-01" @default.
• W2912296833 modified "2023-10-07" @default.
• W2912296833 title "Self-reporting transposons enable simultaneous readout of gene expression and transcription factor binding in single cells" @default.
• W2912296833 cites W1539046593 @default.
• W2912296833 cites W1602702667 @default.
• W2912296833 cites W1859359294 @default.
• W2912296833 cites W1887325245 @default.
• W2912296833 cites W1967983317 @default.
• W2912296833 cites W1971578941 @default.
• W2912296833 cites W1975203903 @default.
• W2912296833 cites W1979283544 @default.
• W2912296833 cites W1980273804 @default.
• W2912296833 cites W1987841739 @default.
• W2912296833 cites W1988375781 @default.
• W2912296833 cites W1993063708 @default.
• W2912296833 cites W2004966039 @default.
• W2912296833 cites W2009869892 @default.
• W2912296833 cites W2011637823 @default.
• W2912296833 cites W2012666288 @default.
• W2912296833 cites W2023186182 @default.
• W2912296833 cites W2026641296 @default.
• W2912296833 cites W2026863186 @default.
• W2912296833 cites W2030110789 @default.
• W2912296833 cites W2031855875 @default.
• W2912296833 cites W2036840727 @default.
• W2912296833 cites W2036897871 @default.
• W2912296833 cites W2051006651 @default.
• W2912296833 cites W2054275187 @default.
• W2912296833 cites W2056997870 @default.
• W2912296833 cites W2060484997 @default.
• W2912296833 cites W2064390432 @default.
• W2912296833 cites W2065236031 @default.
• W2912296833 cites W2073572813 @default.
• W2912296833 cites W2074679317 @default.
• W2912296833 cites W2076166032 @default.
• W2912296833 cites W2076438782 @default.
• W2912296833 cites W2077882710 @default.
• W2912296833 cites W2090461073 @default.
• W2912296833 cites W2099434166 @default.
• W2912296833 cites W2102212449 @default.
• W2912296833 cites W2102619694 @default.
• W2912296833 cites W2107680358 @default.
• W2912296833 cites W2109818073 @default.
• W2912296833 cites W2110387447 @default.
• W2912296833 cites W2117792884 @default.
• W2912296833 cites W2123784922 @default.
• W2912296833 cites W2125987139 @default.
• W2912296833 cites W2126119172 @default.
• W2912296833 cites W2128151873 @default.
• W2912296833 cites W2128589652 @default.
• W2912296833 cites W2128940002 @default.
• W2912296833 cites W2129042241 @default.
• W2912296833 cites W2129795133 @default.
• W2912296833 cites W2137465688 @default.
• W2912296833 cites W2141638818 @default.
• W2912296833 cites W2148896458 @default.
• W2912296833 cites W2152014285 @default.
• W2912296833 cites W2157952888 @default.
• W2912296833 cites W2158771952 @default.
• W2912296833 cites W2164618384 @default.
• W2912296833 cites W2164813466 @default.
• W2912296833 cites W2165890492 @default.
• W2912296833 cites W2171808845 @default.
• W2912296833 cites W2198371822 @default.
• W2912296833 cites W2211837538 @default.
• W2912296833 cites W2222000795 @default.
• W2912296833 cites W2231865019 @default.
• W2912296833 cites W2235841654 @default.
• W2912296833 cites W2237299736 @default.
• W2912296833 cites W2259938310 @default.
• W2912296833 cites W2261494289 @default.
• W2912296833 cites W2341539131 @default.
• W2912296833 cites W2342782366 @default.
• W2912296833 cites W2493663318 @default.
• W2912296833 cites W2536097271 @default.
• W2912296833 cites W2559466477 @default.
• W2912296833 cites W2561754210 @default.
• W2912296833 cites W2576162457 @default.
• W2912296833 cites W2577994754 @default.
• W2912296833 cites W2586421092 @default.
• W2912296833 cites W2594621310 @default.
• W2912296833 cites W2605624585 @default.
• W2912296833 cites W2739492614 @default.
• W2912296833 cites W2747545374 @default.
• W2912296833 cites W2752426001 @default.
• W2912296833 cites W2753908609 @default.
• W2912296833 cites W2759601393 @default.
• W2912296833 cites W2763864472 @default.

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