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• W2912300644 abstract "Galectins (Gals) are a family of animal lectins that bind &beta;-galactosides through a carbohydrate recognition domain. Galectin-8 is a tandem-repeat galectin, secreted intracellularly and extracellularly. It is associated with neutrophil migration and has been studied as a possible therapeutic to combat inflammation. The objective of this study was to evaluate the translational and the transcriptional effects of recombinant Galectin-8 (rGal-8) on cow neutrophils. Blood was collected aseptically from Holstein-Friesian cows (n=10) from the North Carolina A&amp;T State University Dairy Unit. Neutrophils isolated were treated with rGal-8 (2&mu;g), or PBS (control) and were incubated at 37&deg;C, 5% CO2 for 1 hour. Supernatant from treated neutrophils was evaluated for total protein concentration, and galectin-8 secretion using bovine Galectin-8 Enzyme Linked-Immuno-Sorbent Assay (ELISA) kit. Total RNA was extracted, reverse transcribed, and RT-qPCR was performed using the RT&sup2; Profiler Cow Innate &amp; Adaptive Immune Responses Array with 84 genes. The Livak method was used to calculate transcript abundance and fold change (FC&gt;2 considered significant). Total protein concentration increased (P=0.0361) after rGal-8 treatment compared to the untreated control. Galectin-8 secretion was not significantly different in control compared to treated group (P=0.5819). Out of the 84 genes, 81 genes were differentially expressed in response to rGal-8; 14 up-regulated, 5 down-regulated, 61 genes remained unchanged. Treatment with rGal8 induced the expression of IRF7. The top five up-regulated genes include FAS, CD40, CD86, IFNGR1, STAT1; down-regulated genes were TLR9, CD14, CCR6, TICAM1, and TLR1. Selected genes were probed to validate fold change; the levels of gene expression were comparable to data from RT2 array. Exposure of bovine neutrophils to rGal-8 modified expression of immune response genes. The functional significance of the change needs further studies." @default.
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• W2912300644 date "2019-01-29" @default.
• W2912300644 modified "2023-10-17" @default.
• W2912300644 title "Galectin-8 Modulates Innate and Adaptive Immune Response Genes in Bovine Neutrophils" @default.
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• W2912300644 doi "https://doi.org/10.5539/jmbr.v9n1p24" @default.
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