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- W2912304232 abstract "为揭示大头鳕 TNFSF 6基因的基本结构与功能及其在大头鳕发育和神经坏死病毒(Pacific cod nervous necrosis virus, PCNNV)暴发时的响应机制，本研究通过基因克隆获得 TNFSF 6 cDNA开放阅读框序列，并对序列进行生物信息学分析，运用相对荧光定量PCR(qRT-PCR)方法对 TNFSF 6在不同组织、孵化后不同日龄仔鱼和PCNNV感染前后仔稚鱼中的表达水平进行检测。结果显示，大头鳕 TNFSF 6 cDNA长1 388 bp，5′UTR占315 bp，3′UTR占500 bp，ORF全长573 bp，编码190个氨基酸。qRT-PCR结果显示， TNFSF 6在各组织均有表达，但在脾脏和鳃组织中的表达量较高；大头鳕孵化后15、20、37和40 d TNFSF 6基因的转录水平分别是其在5 d转录水平的0.28、0.15、0.12和0.13倍。在24 d和46 d PCNNV暴发时，病鱼 TNFSF 6基因的转录水平高于对照组；在77 d PCNNV暴发时，病鱼 TNFSF 6转录水平则显著低于对照组。研究表明， TNFSF 6基因在大头鳕发育早期和仔鱼暴发PCNNV时发挥了重要的作用。" @default.
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- W2912304232 date "2019-02-25" @default.
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- W2912304232 title "大头鳕 TNFSF 6基因的结构分析及其在发育早期和病毒暴发时的表达水平" @default.
- W2912304232 doi "https://doi.org/10.11964/jfc.20171010995" @default.
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