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- W2912341106 abstract "Abstract The isolation of root nodule bacteria (RNB) usually lead to obtainment of several non-rhizobia, mainly those fast-growing bacteria. The plant-authentication experiments to select the nodulating bacteria are time consuming and can be ruined in case of nodulation of negative controls. To speed up this step in rhizobiology research a fast, simple and efficient duplex PCR assay for the amplification of nodC and nifH genes in was developed aiming to separate rhizobia and non-rhizobia in RNB culture collections. The method was optimized with 43 reference strains and was applied to a new culture collection for validation. Considering all bacteria, 106 out of 109 nodulating rhizobia were positive for both genes. Among non-nodulating, 48 out of 48 were nodC-negative. The results demonstrated the efficiency of this new method that can be completed in a day, to save time and money in RNB isolation projects, fulfilling Koch's postulate, and separating rhizobia from non-rhizobial bacteria." @default.
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- W2912341106 date "2019-06-01" @default.
- W2912341106 modified "2023-10-17" @default.
- W2912341106 title "Fast and efficient symbiotic gene-based duplex PCR approach for the preliminary selection of legume root nodule bacteria" @default.
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