FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912342280> ?p ?o ?g. }

• W2912342280 abstract "Abstract The signal transducer and activator of transcription 3 (STAT3) protein is activated by phosphorylation of a specific tyrosine residue (Tyr705) in response to various extracellular signals. STAT3 activity was also found to be regulated by acetylation of Lys685. However, the molecular mechanism by which Lys685 acetylation affects the transcriptional activity of STAT3 remains elusive. By genetically encoding the co-translational incorporation of acetyl-lysine into position Lys685 and co-expression with the Elk receptor tyrosine kinase, we were able to biochemically characterize site-specifically acetylated, and simultaneously acetylated and phosphorylated STAT3. We measured the effect of acetylation on the crystal structure, and DNA binding affinity and specificity of Tyr705-phosphorylated and non-phosphorylated STAT3. In addition, we monitored the deacetylation of acetylated Lys685 by reconstituting the mammalian enzymatic deacetylation reaction in live bacteria. Surprisingly, we found that acetylation, per se , had no effect on the crystal structure, and DNA binding affinity or specificity of STAT3, implying that the previously observed acetylation-dependent transcriptional activity of STAT3 involves an additional cellular component. In addition, we discovered that Tyr705-phosphorylation protects Lys685 from deacetylation in bacteria, providing a new possible explanation for the observed correlation between STAT3 activity and Lys685 acetylation." @default.
• W2912342280 created "2019-02-21" @default.
• W2912342280 creator A5000601221 @default.
• W2912342280 creator A5015491789 @default.
• W2912342280 creator A5017647216 @default.
• W2912342280 creator A5053265861 @default.
• W2912342280 creator A5055375647 @default.
• W2912342280 creator A5056377039 @default.
• W2912342280 creator A5080924271 @default.
• W2912342280 creator A5082030263 @default.
• W2912342280 creator A5086527390 @default.
• W2912342280 date "2019-02-01" @default.
• W2912342280 modified "2023-09-23" @default.
• W2912342280 title "Unexpected implications of STAT3 acetylation revealed by genetic encoding of acetyl-lysine" @default.
• W2912342280 cites W1965398762 @default.
• W2912342280 cites W1975567748 @default.
• W2912342280 cites W1981799488 @default.
• W2912342280 cites W1987944461 @default.
• W2912342280 cites W1994122295 @default.
• W2912342280 cites W2020238484 @default.
• W2912342280 cites W2023546541 @default.
• W2912342280 cites W2027253055 @default.
• W2912342280 cites W2042503285 @default.
• W2912342280 cites W2042520699 @default.
• W2912342280 cites W2044227662 @default.
• W2912342280 cites W2059127283 @default.
• W2912342280 cites W2072669480 @default.
• W2912342280 cites W2073189570 @default.
• W2912342280 cites W2074356752 @default.
• W2912342280 cites W2078929930 @default.
• W2912342280 cites W2083802827 @default.
• W2912342280 cites W2084100857 @default.
• W2912342280 cites W2086473824 @default.
• W2912342280 cites W2089502862 @default.
• W2912342280 cites W2093155739 @default.
• W2912342280 cites W2103422355 @default.
• W2912342280 cites W2107167859 @default.
• W2912342280 cites W2112716343 @default.
• W2912342280 cites W2123727040 @default.
• W2912342280 cites W2148123458 @default.
• W2912342280 cites W2148313902 @default.
• W2912342280 cites W2149070581 @default.
• W2912342280 cites W2149769193 @default.
• W2912342280 cites W2151850046 @default.
• W2912342280 cites W2153231604 @default.
• W2912342280 cites W2159268679 @default.
• W2912342280 cites W2169636286 @default.
• W2912342280 cites W2565236299 @default.
• W2912342280 cites W2610072177 @default.
• W2912342280 cites W2783142245 @default.
• W2912342280 cites W2799941631 @default.
• W2912342280 cites W2834541610 @default.
• W2912342280 cites W2884133005 @default.
• W2912342280 cites W2889958470 @default.
• W2912342280 cites W477780388 @default.
• W2912342280 doi "https://doi.org/10.1101/537696" @default.
• W2912342280 hasPublicationYear "2019" @default.
• W2912342280 type Work @default.
• W2912342280 sameAs 2912342280 @default.
• W2912342280 citedByCount "0" @default.
• W2912342280 crossrefType "posted-content" @default.
• W2912342280 hasAuthorship W2912342280A5000601221 @default.
• W2912342280 hasAuthorship W2912342280A5015491789 @default.
• W2912342280 hasAuthorship W2912342280A5017647216 @default.
• W2912342280 hasAuthorship W2912342280A5053265861 @default.
• W2912342280 hasAuthorship W2912342280A5055375647 @default.
• W2912342280 hasAuthorship W2912342280A5056377039 @default.
• W2912342280 hasAuthorship W2912342280A5080924271 @default.
• W2912342280 hasAuthorship W2912342280A5082030263 @default.
• W2912342280 hasAuthorship W2912342280A5086527390 @default.
• W2912342280 hasBestOaLocation W29123422801 @default.
• W2912342280 hasConcept C104317684 @default.
• W2912342280 hasConcept C119157956 @default.
• W2912342280 hasConcept C11960822 @default.
• W2912342280 hasConcept C153911025 @default.
• W2912342280 hasConcept C185592680 @default.
• W2912342280 hasConcept C2776016237 @default.
• W2912342280 hasConcept C2776165026 @default.
• W2912342280 hasConcept C2777553839 @default.
• W2912342280 hasConcept C515207424 @default.
• W2912342280 hasConcept C55493867 @default.
• W2912342280 hasConcept C86803240 @default.
• W2912342280 hasConcept C95444343 @default.
• W2912342280 hasConceptScore W2912342280C104317684 @default.
• W2912342280 hasConceptScore W2912342280C119157956 @default.
• W2912342280 hasConceptScore W2912342280C11960822 @default.
• W2912342280 hasConceptScore W2912342280C153911025 @default.
• W2912342280 hasConceptScore W2912342280C185592680 @default.
• W2912342280 hasConceptScore W2912342280C2776016237 @default.
• W2912342280 hasConceptScore W2912342280C2776165026 @default.
• W2912342280 hasConceptScore W2912342280C2777553839 @default.
• W2912342280 hasConceptScore W2912342280C515207424 @default.
• W2912342280 hasConceptScore W2912342280C55493867 @default.
• W2912342280 hasConceptScore W2912342280C86803240 @default.
• W2912342280 hasConceptScore W2912342280C95444343 @default.
• W2912342280 hasLocation W29123422801 @default.
• W2912342280 hasOpenAccess W2912342280 @default.
• W2912342280 hasPrimaryLocation W29123422801 @default.
• W2912342280 hasRelatedWork W1562710579 @default.
• W2912342280 hasRelatedWork W2046293919 @default.

• next