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• W2912342283 abstract "Abstract First, a protocol for increasing the stoichiometry of O‐GlcNAc on proteins is given. This is followed by simple techniques for the detection/screening of O‐GlcNAc‐modified proteins either by immunoblotting or lectin affinity chromatography. Separate protocols verify that the glycan is O‐linked GlcNAc. These methods are followed by protocols for more comprehensive analysis of O‐GlcNAc modified proteins, including labeling of O‐GlcNAc residues with [3H]Gal, and subsequent product analysis. The final two protocols assay for O‐GlcNAc transferase and O‐GlcNAcase activity, respectively." @default.
• W2912342283 created "2019-02-21" @default.
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• W2912342283 date "2001-09-01" @default.
• W2912342283 modified "2023-10-16" @default.
• W2912342283 title "Detection and Analysis of Proteins Modified by O‐Linked N‐Acetylglucosamine" @default.
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• W2912342283 doi "https://doi.org/10.1002/0471140864.ps1208s25" @default.
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