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- W2912349041 abstract "Torpedo-stage somatic embryos were selected from actively growing cultures and trasferred to embryo maintenance medium [MS with (per liter) 412.5 mg NH 4 NO 3 , 475 mg KNO 3 , 1 g myo -inositol, 90 g sucrose, 2 g activated charcoal, and 7 g TC agar] supplemented with either 0%, 2.5%, 5%, 7.5%, or 10% polyethylene glycol (PEG) for 4, 8, or 12 weeks. Embryos placed on treatment media were transferred directly to grape somatic embryo germination medium [MS with (per liter) 1 g myo -inositol, 30 g sucrose, 1 M benzyladenine, and 7 g TC agar] once their PEG treatment was terminated. The number of embryos that germinated was recorded 4 weeks after transfer to somatic embryo germination medium. The number of germinated embryos that differentiated into plants was recorded at 8 weeks. There was no difference in germination rates and embryo differentiation among embryos incubated on medium with or without PEG for 4 weeks. A difference in embryo growth rate was observed after 8 weeks on medium with PEG. Embryo grew fastest on media containing 5% or 7.5% PEG. In addition, embryos grown on medium with 5% or 7.5% PEG were morphologically similar to zygotic embryos." @default.
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- W2912349041 date "1998-06-01" @default.
- W2912349041 modified "2023-10-17" @default.
- W2912349041 title "Effects of Polyethylene Glycol on Development of Grape (Vitis vinifera L. `Thompson Seedless') Somatic Embryos" @default.
- W2912349041 doi "https://doi.org/10.21273/hortsci.33.3.557c" @default.
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