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• W2912349547 abstract "The meiotic recombination checkpoint blocks meiotic cell cycle progression in response to synapsis and/or recombination defects to prevent aberrant chromosome segregation. The evolutionarily conserved budding yeast Pch2TRIP13 AAA+ ATPase participates in this pathway by supporting phosphorylation of the Hop1HORMAD adaptor at T318. In the wild type, Pch2 localizes to synapsed chromosomes and to the unsynapsed rDNA region (nucleolus), excluding Hop1. In contrast, in synaptonemal complex (SC)–defective zip1Δ mutants, which undergo checkpoint activation, Pch2 is detected only on the nucleolus. Alterations in some epigenetic marks that lead to Pch2 dispersion from the nucleolus suppress zip1Δ-induced checkpoint arrest. These observations have led to the notion that Pch2 nucleolar localization could be important for the meiotic recombination checkpoint. Here we investigate how Pch2 chromosomal distribution impacts checkpoint function. We have generated and characterized several mutations that alter Pch2 localization pattern resulting in aberrant Hop1 distribution and compromised meiotic checkpoint response. Besides the AAA+ signature, we have identified a basic motif in the extended N-terminal domain critical for Pch2’s checkpoint function and localization. We have also examined the functional relevance of the described Orc1-Pch2 interaction. Both proteins colocalize in the rDNA, and Orc1 depletion during meiotic prophase prevents Pch2 targeting to the rDNA allowing unwanted Hop1 accumulation on this region. However, Pch2 association with SC components remains intact in the absence of Orc1. We finally show that checkpoint activation is not affected by the lack of Orc1 demonstrating that, in contrast to previous hypotheses, nucleolar localization of Pch2 is actually dispensable for the meiotic checkpoint." @default.
• W2912349547 created "2019-02-21" @default.
• W2912349547 creator A5002349969 @default.
• W2912349547 creator A5023707352 @default.
• W2912349547 creator A5059275122 @default.
• W2912349547 creator A5079414446 @default.
• W2912349547 creator A5083487166 @default.
• W2912349547 date "2019-03-12" @default.
• W2912349547 modified "2023-10-03" @default.
• W2912349547 title "Characterization of Pch2 localization determinants reveals a nucleolar-independent role in the meiotic recombination checkpoint" @default.
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• W2912349547 doi "https://doi.org/10.1007/s00412-019-00696-7" @default.
• W2912349547 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30859296" @default.
• W2912349547 hasPublicationYear "2019" @default.
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