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- W2912373639 abstract "Abstract Cilia and flagella are long, slender organelles found in many eukaryotic cells where they have sensory, developmental and motile functions. All cilia and flagella contain a microtubule-based structure called the axoneme. In motile cilia and flagella, which drive cell locomotion and fluid transport, the axoneme contains, along most of its length, motor proteins from the axonemal dynein family. These motor proteins drive motility by using energy derived from the hydrolysis of ATP to generate a bending wave, which travels down the axoneme. As a first step towards visualizing the ATPase activity of the axonemal dyneins during bending, we have investigated the kinetics of nucleotide binding to axonemes. Using a specially built UV-TIRF microscope, we found that the fluorescent ATP analog mantATP (methylanthraniloyl adenosine triphosphate), which has been shown to support axonemal motility, binds all along isolated, immobilized axonemes. By studying the recovery of fluorescence after photobleaching, we found that there are three mantATP binding sites: one that bleaches rapidly (time constant ≈ 1.7 s) and recovers slowly (time constant ≈ 44 s), one that bleaches with the same time constant but does not recover and one that does not bleach. By reducing the dynein content in the axoneme using mutants and salt extraction, we provide evidence that the slow-recovering component corresponds to axonemal dyneins. The recovery rate of this component, however, is too slow to be consistent with the activation of beating observed at higher mantATP concentrations; this indicates that the dyneins may be inhibited due to their immobilization at the surface. The development of this method is a first step towards direct observation of the travelling wave of dynein activity." @default.
- W2912373639 created "2019-02-21" @default.
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- W2912373639 date "2019-02-11" @default.
- W2912373639 modified "2023-09-23" @default.
- W2912373639 title "The kinetics of nucleotide binding to isolated<i>Chlamydomonas</i>axonemes using UV-TIRF microscopy" @default.
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- W2912373639 doi "https://doi.org/10.1101/547091" @default.
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