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- W2912378009 abstract "PKCβI, a member of the classical protein kinase C family, plays key roles in regulating cell cycle transition. Here, we report the expression, localization and functions of PKCβI in mouse oocyte meiotic maturation. PKCβI and p-PKCβI (phosphor-PKCβI) were expressed from germinal vesicle (GV) stage to metaphase II (MII) stage. Confocal microscopy revealed that PKCβI was localized in the GV and evenly distributed in the cytoplasm after GV breakdown (GVBD), and it was concentrated at the midbody at telophase in meiotic oocytes. While, p-PKCβI was concentrated at the spindle poles at the metaphase stages and associated with midbody at telophase. Depletion of PKCβI by specific siRNA injection resulted in defective spindles, accompanied with spindle assembly checkpoint activation, metaphase I arrest and failure of first polar body (PB1) extrusion. Live cell imaging analysis also revealed that knockdown of PKCβI resulted in abnormal spindles, misaligned chromosomes, and meiotic arrest of oocytes arrest at the Pro-MI/MI stage. PKCβI depletion did not affect the G2/M transition, but its overexpression delayed the G2/M transition through regulating Cyclin B1 level and Cdc2 activity. Our findings reveal that PKCβI is a critical regulator of meiotic cell cycle progression in oocytes. Abbreviations: PKC, protein kinase C; COC, cumulus-oocyte complexes; GV, germinal vesicle; GVBD, germinal vesicle breakdown; Pro-MI, first pro-metaphase; MI, first metaphase; Tel I, telophase I; MII, second metaphase; PB1, first polar body; SAC, spindle assembly checkpoint." @default.
- W2912378009 created "2019-02-21" @default.
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- W2912378009 date "2019-02-07" @default.
- W2912378009 modified "2023-09-26" @default.
- W2912378009 title "PKCβ1 regulates meiotic cell cycle in mouse oocyte" @default.
- W2912378009 cites W1486461179 @default.
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- W2912378009 cites W1532445586 @default.
- W2912378009 cites W1868128271 @default.
- W2912378009 cites W1967459937 @default.
- W2912378009 cites W1969315619 @default.
- W2912378009 cites W1972307267 @default.
- W2912378009 cites W1976129687 @default.
- W2912378009 cites W1978592193 @default.
- W2912378009 cites W1979946750 @default.
- W2912378009 cites W1993107494 @default.
- W2912378009 cites W2001486236 @default.
- W2912378009 cites W2004325651 @default.
- W2912378009 cites W2005131330 @default.
- W2912378009 cites W2005682259 @default.
- W2912378009 cites W2021148581 @default.
- W2912378009 cites W2027066789 @default.
- W2912378009 cites W2027279149 @default.
- W2912378009 cites W2028117036 @default.
- W2912378009 cites W2034480539 @default.
- W2912378009 cites W2036932956 @default.
- W2912378009 cites W2038297783 @default.
- W2912378009 cites W2041992758 @default.
- W2912378009 cites W2042082556 @default.
- W2912378009 cites W2043052985 @default.
- W2912378009 cites W2043367150 @default.
- W2912378009 cites W2049616423 @default.
- W2912378009 cites W2061842603 @default.
- W2912378009 cites W2065305930 @default.
- W2912378009 cites W2071731130 @default.
- W2912378009 cites W2072513809 @default.
- W2912378009 cites W2075152619 @default.
- W2912378009 cites W2076606495 @default.
- W2912378009 cites W2079019214 @default.
- W2912378009 cites W2084344228 @default.
- W2912378009 cites W2087941042 @default.
- W2912378009 cites W2088322370 @default.
- W2912378009 cites W2093814864 @default.
- W2912378009 cites W2097184870 @default.
- W2912378009 cites W2097287838 @default.
- W2912378009 cites W2101694285 @default.
- W2912378009 cites W2104969843 @default.
- W2912378009 cites W2106871348 @default.
- W2912378009 cites W2113651151 @default.
- W2912378009 cites W2115343462 @default.
- W2912378009 cites W2123075417 @default.
- W2912378009 cites W2123488059 @default.
- W2912378009 cites W2127397312 @default.
- W2912378009 cites W2130208142 @default.
- W2912378009 cites W2134555179 @default.
- W2912378009 cites W2134810944 @default.
- W2912378009 cites W2141300577 @default.
- W2912378009 cites W2152752168 @default.
- W2912378009 cites W2157330568 @default.
- W2912378009 cites W2159177242 @default.
- W2912378009 cites W2163585955 @default.
- W2912378009 cites W2171405627 @default.
- W2912378009 cites W2176482710 @default.
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- W2912378009 cites W2239802822 @default.
- W2912378009 cites W2434541077 @default.
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- W2912378009 doi "https://doi.org/10.1080/15384101.2018.1564492" @default.
- W2912378009 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6422498" @default.
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