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- W2912378221 abstract "An improved medium for the in-vitro micropropagation of P. vera genotypes was developed. Several basal medium preparations were tested and DKW (Driver-Kuniyuki-Walnut) medium was selected. Macro and micronutrients were adjusted to provide optimum growth and multiplication of shoots, some of which have been grown and multiplied for more than 2 years. The use of thidiazuron as a growth regulator was tested, but was detrimental to the explants at all tested concentrations. Initial experiments were hindered by significant bacterial internal contaminants. This problem was eliminated through the use of plexiglass chambers to provide up to a 20,000ppm CO2 atmosphere, increased light levels to promote photosynthesis, and the elimination of all carbon sources (sugars) from the substrate. An additional benefit was better shoot growth, better survival when rooting and better acclimation. 3/7 plants were rooted using 2.5 ppm IBA. Continuing experiments are focused on the effect of support medium on growth and multiplication as well as media development for other Pistacia species." @default.
- W2912378221 created "2019-02-21" @default.
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- W2912378221 date "1992-06-01" @default.
- W2912378221 modified "2023-09-23" @default.
- W2912378221 title "USE OF A HIGH CO2 ATMOSPHERE FOR MICROPROPAGATION OF PISTACHIO" @default.
- W2912378221 doi "https://doi.org/10.21273/hortsci.27.6.697e" @default.
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