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• W2912382675 startingPage "31" @default.
• W2912382675 abstract "We report on the variable venom composition of a population of the Caucasus viper (Vipera kaznakovi) in Northeastern Turkey. We applied a combination of venom gland transcriptomics, de-complexing bottom-up and top-down venomics. In contrast to sole bottom-up venomics approaches and gel or chromatography based venom comparison, our combined approach enables a faster and more detailed comparison of venom proteomes from multiple individuals. In total, we identified peptides and proteins from 15 toxin families, including snake venom metalloproteinases (svMP; 37.8%), phospholipases A2 (PLA2; 19.0%), snake venom serine proteinases (svSP; 11.5%), C-type lectins (CTL; 6.9%) and cysteine-rich secretory proteins (CRISP; 5.0%), in addition to several low abundant toxin families. Furthermore, we identified intraspecies variations of the venom composition of V. kaznakovi, and find these were mainly driven by the age of the animals, with lower svSP abundance detected in juveniles. On the proteoform level, several small molecular weight toxins between 5 and 8 kDa in size, as well as PLA2s, drove the differences observed between juvenile and adult individuals. This study provides novel insights into the venom variability of V. kaznakovi and highlights the utility of intact mass profiling for fast and detailed comparison of snake venom. Population level and ontogenetic venom variation (e.g. diet, habitat, sex or age) can result in a loss of antivenom efficacy against snakebites from wide ranging snake populations. The current state of the art for the analysis of snake venoms are de-complexing bottom-up proteomics approaches. While useful, these have the significant drawback of being time-consuming and following costly protocols, and consequently are often applied to pooled venom samples. To overcome these shortcomings and to enable rapid and detailed profiling of large numbers of individual venom samples, we integrated an intact protein analysis workflow into a transcriptomics-guided bottom-up approach. The application of this workflow to snake individuals of a local population of V. kaznakovi revealed intraspecies variations in venom composition, which are primarily explained by the age of the animals, and highlighted svSP abundance to be one of the molecular drivers for the compositional differences observed." @default.
• W2912382675 created "2019-02-21" @default.
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• W2912382675 date "2019-05-01" @default.
• W2912382675 modified "2023-10-03" @default.
• W2912382675 title "Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey" @default.
• W2912382675 cites W1017783917 @default.
• W2912382675 cites W1565563859 @default.
• W2912382675 cites W1568341953 @default.
• W2912382675 cites W1645500705 @default.
• W2912382675 cites W1844957996 @default.
• W2912382675 cites W1890259938 @default.
• W2912382675 cites W1966881658 @default.
• W2912382675 cites W1968941215 @default.
• W2912382675 cites W1970187222 @default.
• W2912382675 cites W1973268693 @default.
• W2912382675 cites W1974116356 @default.
• W2912382675 cites W1977027334 @default.
• W2912382675 cites W1978639305 @default.
• W2912382675 cites W1981120493 @default.
• W2912382675 cites W1981733318 @default.
• W2912382675 cites W1983678954 @default.
• W2912382675 cites W1992599449 @default.
• W2912382675 cites W2007970752 @default.
• W2912382675 cites W2016916625 @default.
• W2912382675 cites W2022401530 @default.
• W2912382675 cites W2023010129 @default.
• W2912382675 cites W2026578694 @default.
• W2912382675 cites W2027220432 @default.
• W2912382675 cites W2027898785 @default.
• W2912382675 cites W2031952431 @default.
• W2912382675 cites W2037717176 @default.
• W2912382675 cites W2042987909 @default.
• W2912382675 cites W2044173628 @default.
• W2912382675 cites W2046003233 @default.
• W2912382675 cites W2053997108 @default.
• W2912382675 cites W2055043387 @default.
• W2912382675 cites W2056268524 @default.
• W2912382675 cites W2057326625 @default.
• W2912382675 cites W2061200975 @default.
• W2912382675 cites W2066489282 @default.
• W2912382675 cites W2077905837 @default.
• W2912382675 cites W2080967293 @default.
• W2912382675 cites W2103546835 @default.
• W2912382675 cites W210522119 @default.
• W2912382675 cites W2118303674 @default.
• W2912382675 cites W2135995399 @default.
• W2912382675 cites W2143162691 @default.
• W2912382675 cites W2143483161 @default.
• W2912382675 cites W2144712355 @default.
• W2912382675 cites W2150146437 @default.
• W2912382675 cites W2150925001 @default.
• W2912382675 cites W2155094655 @default.
• W2912382675 cites W2155443196 @default.
• W2912382675 cites W2156479756 @default.
• W2912382675 cites W2156818122 @default.
• W2912382675 cites W2163916590 @default.
• W2912382675 cites W2229266328 @default.
• W2912382675 cites W2321769818 @default.
• W2912382675 cites W2326188727 @default.
• W2912382675 cites W2339628848 @default.
• W2912382675 cites W2378145088 @default.
• W2912382675 cites W2396726851 @default.
• W2912382675 cites W2434026737 @default.
• W2912382675 cites W2461049151 @default.
• W2912382675 cites W2581733574 @default.
• W2912382675 cites W2589845476 @default.
• W2912382675 cites W2609099285 @default.
• W2912382675 cites W2609651208 @default.
• W2912382675 cites W2623643269 @default.
• W2912382675 cites W2624721949 @default.
• W2912382675 cites W2737146600 @default.
• W2912382675 cites W2745568778 @default.
• W2912382675 cites W2754620869 @default.
• W2912382675 cites W2759785651 @default.
• W2912382675 cites W2781225082 @default.
• W2912382675 cites W2781973335 @default.
• W2912382675 cites W2800248379 @default.
• W2912382675 cites W2803614773 @default.
• W2912382675 cites W2809440213 @default.
• W2912382675 cites W2825746406 @default.
• W2912382675 cites W2883645137 @default.
• W2912382675 cites W2883995122 @default.
• W2912382675 cites W2884269524 @default.
• W2912382675 cites W2887673377 @default.
• W2912382675 cites W2899424351 @default.
• W2912382675 cites W4240535283 @default.
• W2912382675 cites W4244141504 @default.
• W2912382675 cites W4376453191 @default.

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