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- W2912383364 abstract "Serotonin is involved in the regulation of many physiological and behavioral processes in vertebrates and invertebrates. The effects of serotonin are mediated through interactions of several 5-HT receptor types. The expression and pharmacological properties of 5-HT1 have received more attention than other serotonin receptors, but its functions at the individual level are little studied in arthropods. Silkworm, a Lepidoptera model, almost has no reports about serotonin receptors. To analyze the function of Bm5-HT1A receptor in vitro, the ORF of Bm5-HT1A was cloned into the pcDNA3.1 vector and expressed in HEK 293 cells. Serotonin activation of Bm5-HT1A-expressing cells decreased forskolin-stimulated cAMP synthesis and had the most potent effect compared to other biogenic amines. Serotonin reduced cAMP synthesis in a dose-dependent manner, and half-maximal activation (EC50) occurred at a concentration of 1.17 × 10−7 M (117 nM). The pharmacological analysis demonstrated that the rank potency of agonists was pimozide >8-OH-DPAT >5-MeOT ~ αm-5-HT, and antagonists was WAY-100635 > prazosin > SB-269970 > methiothepin at the Bm5-HT1A receptor. Injecting the antagonist of Bm5-HT1A receptor into larvae caused slow or weak motility, and adults lowered courtship vitality or moving speed. Injecting dsRNA of Bm5-HT1A into adults also dropped locomotivity in courtship. These results show that the Bm5-HT1A receptor is related to locomotor activity. This study provides the first information of serotonin receptor on pharmacological in silkworm and on individual functions in arthropods." @default.
- W2912383364 created "2019-02-21" @default.
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- W2912383364 date "2019-05-01" @default.
- W2912383364 modified "2023-10-15" @default.
- W2912383364 title "Pharmacological characterization of the 5-HT1A receptor of Bombyx mori and its role in locomotion" @default.
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- W2912383364 doi "https://doi.org/10.1016/j.cbpa.2019.01.020" @default.
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