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• W2912390034 startingPage "196" @default.
• W2912390034 abstract "3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1." @default.
• W2912390034 created "2019-02-21" @default.
• W2912390034 creator A5010359979 @default.
• W2912390034 creator A5034889937 @default.
• W2912390034 date "2019-03-04" @default.
• W2912390034 modified "2023-10-03" @default.
• W2912390034 title "Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains" @default.
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• W2912390034 doi "https://doi.org/10.3390/genes10030196" @default.
• W2912390034 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6471042" @default.
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• W2912390034 hasPublicationYear "2019" @default.
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