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- W2912390141 abstract "Introduction:Helicobacter pylori infection is a risk factor for gastritis, ulcers and gastric cancer. H. pylori's most studied virulence factors are vacuolating cytotoxin A (Vac A) and the cagA. There are very few studies on cagA gene detection comparing high and low lands in the Andean region. It has been reported that signal sequence of vacA gene (s) is a major virulence factor in occident whereas in East Asia the middle region (m) of the same gen is the most toxigenic. The combination of cagA and vac s1/m1 is most associated with gastric cancer development. The aim of this study was to genotype H. pylori attached to gastric mucosa of dyspeptic patients from Quito and Esmeraldas and establish the probable relationship between the genotype and the geographical population distribution. Methods: A cross-sectional study was conducted from March to June 2013 in Esmeraldas (E; sea level) and Quito (Q; 2 869 meters above sea level). Eighty patients with dyspepsia, 18-75 years of age (E n=30, Q n=50), not taking pump inhibitors or antibiotics were studied. Gastric biopsies were obtained per the Sydney method. Conventional tissue processing was performed for histopathology analysis. Five tissue samples were arrayed in individual paraffin blocks, laser microdissection was used to select attached bacteria to mucus layer in gastric tissue and real-time PCR were used for detection of cagA, and genotyping of allelic variants of vacA genes. Results: Based on histopathology analysis, 22 samples with moderate (++) or high (+++) H. pylori infection were selected (Q n=14, E n=8). Eighteen samples were suitable for molecular detection based on the amplification of 16s rDNA (Q n=12, E n=6). Samples from Q showed cagA but not vacA amplification in two patients; cagA/vacAs1 in two patients and vacAs1 in one patient. The remaining samples (7) from Q did not amplified the 16s rDNA. Three samples from E patients presented cagA/vacAs2m2, one cagA/vacAs1m2, one cagA/vacAm1 and one vacAs1(n=1). DNA isolation and genotyping were repeated to confirm these results. Conclusion: The most common genes of pathogenicity in Esmeraldas were the cagA/vacAs2m2 which have commonly been reported in Brazil, Chile and Peru. These genes have been considered of intermediate virulence. Whereas in Quito, the most common genes of pathogenicity were cagA (+); vacAs1 strains which have been considered of high virulence. The important proportion of samples from Quito that did not amplify is a limitation of this study." @default.
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- W2912390141 date "2017-10-01" @default.
- W2912390141 modified "2023-09-27" @default.
- W2912390141 title "Different Virulence Genotypes of H. pylori in Dyspeptic Patients From Highlands and Lowlands in Ecuador" @default.
- W2912390141 doi "https://doi.org/10.14309/00000434-201710001-02799" @default.
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