FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912396494> ?p ?o ?g. }

• W2912396494 endingPage "1559" @default.
• W2912396494 startingPage "1549" @default.
• W2912396494 abstract "We report the development of a fast and accurate fluorescence-based assay for amidine linked to cellulose membranes and Sepharose gel. The assay is founded on the glyoxal reaction, which involves reaction of an amidine group with glyoxal and an aromatic aldehyde, leading to the formation of a fluorophore that can be analyzed and quantified by fluorescence spectroscopy and imaging. While the assay has been reported previously for aromatic amidine estimation in solution phase, here we describe its adaptation and application to amidine linked to diverse forms of solid matrices, particularly benzamidine Sepharose and benzamidine-linked cellulose membranes. These functionalized porous matrices find important application in purification of serine proteases. The efficacy of a protein separation device is determined by, among other factors, the ligand (amidine) density. Hence, a sensitive and reproducible method for amidine quantitation in solid phase is needed. The glyoxal reaction was carried out on microbead-sized Sepharose gel and cellulose membranes. Calibration curves were developed for each phase, which established linearity in the range of 0-0.45 μmol per mL amidine for free amidine in solution, 0-0.45 μmol amidine per mL Sepharose gel, and 0-0.48 μmol per mL cellulose membrane. The assay showed high accuracy (~ 3.4% error), precision (RSD < 2%), and reproducibility. Finally, we show how this fluorescent labeling (glyoxal) method can provide a tool for imaging membranes and ligand distribution through confocal laser scanning microscopy. Graphical abstract." @default.
• W2912396494 created "2019-02-21" @default.
• W2912396494 creator A5002250921 @default.
• W2912396494 creator A5016190943 @default.
• W2912396494 creator A5051503177 @default.
• W2912396494 creator A5060062534 @default.
• W2912396494 creator A5065556084 @default.
• W2912396494 creator A5069032140 @default.
• W2912396494 creator A5070277095 @default.
• W2912396494 creator A5078023304 @default.
• W2912396494 creator A5078850234 @default.
• W2912396494 creator A5090387832 @default.
• W2912396494 date "2019-01-24" @default.
• W2912396494 modified "2023-10-06" @default.
• W2912396494 title "In situ analysis and imaging of aromatic amidine at varying ligand densities in solid phase" @default.
• W2912396494 cites W1576562533 @default.
• W2912396494 cites W1598614202 @default.
• W2912396494 cites W1600536828 @default.
• W2912396494 cites W1945974216 @default.
• W2912396494 cites W1976659956 @default.
• W2912396494 cites W1980214070 @default.
• W2912396494 cites W2004368407 @default.
• W2912396494 cites W2012062554 @default.
• W2912396494 cites W2014563853 @default.
• W2912396494 cites W2021966765 @default.
• W2912396494 cites W2022346360 @default.
• W2912396494 cites W2023572749 @default.
• W2912396494 cites W2029950347 @default.
• W2912396494 cites W2034914828 @default.
• W2912396494 cites W2038464633 @default.
• W2912396494 cites W2040080388 @default.
• W2912396494 cites W2056877102 @default.
• W2912396494 cites W2059994721 @default.
• W2912396494 cites W2071641979 @default.
• W2912396494 cites W2074793290 @default.
• W2912396494 cites W2077697332 @default.
• W2912396494 cites W2124733101 @default.
• W2912396494 cites W2126171653 @default.
• W2912396494 cites W2130223248 @default.
• W2912396494 cites W2137447571 @default.
• W2912396494 cites W2146367394 @default.
• W2912396494 cites W2156755609 @default.
• W2912396494 cites W2167279371 @default.
• W2912396494 cites W2186708188 @default.
• W2912396494 cites W2218287097 @default.
• W2912396494 cites W2235341768 @default.
• W2912396494 cites W2260508166 @default.
• W2912396494 cites W2310950758 @default.
• W2912396494 cites W2329556521 @default.
• W2912396494 cites W2330886775 @default.
• W2912396494 cites W2478410551 @default.
• W2912396494 cites W2580035316 @default.
• W2912396494 cites W2593382795 @default.
• W2912396494 cites W2642522075 @default.
• W2912396494 cites W2679333672 @default.
• W2912396494 cites W2736668271 @default.
• W2912396494 cites W4240191929 @default.
• W2912396494 cites W561187754 @default.
• W2912396494 cites W2081270882 @default.
• W2912396494 doi "https://doi.org/10.1007/s00216-019-01588-6" @default.
• W2912396494 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6450742" @default.
• W2912396494 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30675629" @default.
• W2912396494 hasPublicationYear "2019" @default.
• W2912396494 type Work @default.
• W2912396494 sameAs 2912396494 @default.
• W2912396494 citedByCount "0" @default.
• W2912396494 crossrefType "journal-article" @default.
• W2912396494 hasAuthorship W2912396494A5002250921 @default.
• W2912396494 hasAuthorship W2912396494A5016190943 @default.
• W2912396494 hasAuthorship W2912396494A5051503177 @default.
• W2912396494 hasAuthorship W2912396494A5060062534 @default.
• W2912396494 hasAuthorship W2912396494A5065556084 @default.
• W2912396494 hasAuthorship W2912396494A5069032140 @default.
• W2912396494 hasAuthorship W2912396494A5070277095 @default.
• W2912396494 hasAuthorship W2912396494A5078023304 @default.
• W2912396494 hasAuthorship W2912396494A5078850234 @default.
• W2912396494 hasAuthorship W2912396494A5090387832 @default.
• W2912396494 hasBestOaLocation W29123964942 @default.
• W2912396494 hasConcept C178790620 @default.
• W2912396494 hasConcept C181199279 @default.
• W2912396494 hasConcept C185592680 @default.
• W2912396494 hasConcept C2777603720 @default.
• W2912396494 hasConcept C2778622868 @default.
• W2912396494 hasConcept C2779288520 @default.
• W2912396494 hasConcept C41625074 @default.
• W2912396494 hasConcept C43617362 @default.
• W2912396494 hasConcept C55493867 @default.
• W2912396494 hasConceptScore W2912396494C178790620 @default.
• W2912396494 hasConceptScore W2912396494C181199279 @default.
• W2912396494 hasConceptScore W2912396494C185592680 @default.
• W2912396494 hasConceptScore W2912396494C2777603720 @default.
• W2912396494 hasConceptScore W2912396494C2778622868 @default.
• W2912396494 hasConceptScore W2912396494C2779288520 @default.
• W2912396494 hasConceptScore W2912396494C41625074 @default.
• W2912396494 hasConceptScore W2912396494C43617362 @default.
• W2912396494 hasConceptScore W2912396494C55493867 @default.
• W2912396494 hasFunder F4320337354 @default.
• W2912396494 hasFunder F4320337367 @default.

• next