FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912406050> ?p ?o ?g. }

• W2912406050 endingPage "1439" @default.
• W2912406050 startingPage "1425" @default.
• W2912406050 abstract "Genome-wide expression studies using microarrays and RNAseq have increased our understanding of colorectal cancer development. Translating potential gene biomarkers from these studies for clinical utility has typically relied on PCR-based technology and immunohistochemistry. Results from these techniques are limited by tumour sample heterogeneity and the lack of correlation between mRNA transcript abundance and corresponding protein levels. The aim of this research was to investigate the clinical utility of the RNA in situ hybridisation technique, RNAscope®, for measuring intra-tumoural gene expression of potential prognostic markers in a colorectal cancer cohort. Two candidate gene markers (GFI1 and TNFRSF11A) assessed in this study were identified from a previous study led by the The Cancer Genome Atlas (TCGA) Network, and analysis was performed on 112 consecutively collected, archival FFPE colorectal cancer tumour samples. Consistent with the TCGA Network study, we found reduced GFI1 expression was associated with high-grade and left-sided tumours, and reduced TNFRSF11A expression was associated with metastasis and high nodal involvement. RNAscope® combined with image analysis also enabled quantification of GFI1 and TNFRSF11A mRNA expression levels at the single cell level, allowing cell-type determination. These data showed that reduced mRNA transcript abundance measured in patients with poorer prognosis occurred in carcinoma cells, and not lymphocytes, stromal cells or normal epithelial cells. To our knowledge, this is the first study to assess the intra-tumoural expression patterns of GFI1 and TNFRSF11A and to validate their microarray expression profiles using RNAscope. We also demonstrate the utility of RNAscope® technology to show that expression differences are derived from carcinoma cells rather than from cells located in the tumour microenvironment." @default.
• W2912406050 created "2019-02-21" @default.
• W2912406050 creator A5012988855 @default.
• W2912406050 creator A5020154971 @default.
• W2912406050 creator A5038948324 @default.
• W2912406050 creator A5057750122 @default.
• W2912406050 creator A5071809519 @default.
• W2912406050 creator A5079464303 @default.
• W2912406050 creator A5091355914 @default.
• W2912406050 date "2019-02-15" @default.
• W2912406050 modified "2023-09-27" @default.
• W2912406050 title "Assessment of intra-tumoural colorectal cancer prognostic biomarkers using RNA<i>in situ</i>hybridisation" @default.
• W2912406050 cites W1524992328 @default.
• W2912406050 cites W1960895270 @default.
• W2912406050 cites W1973090953 @default.
• W2912406050 cites W1983019576 @default.
• W2912406050 cites W1997700247 @default.
• W2912406050 cites W2001609376 @default.
• W2912406050 cites W2020632674 @default.
• W2912406050 cites W2033544246 @default.
• W2912406050 cites W2038143310 @default.
• W2912406050 cites W2044359460 @default.
• W2912406050 cites W2053368085 @default.
• W2912406050 cites W2065329423 @default.
• W2912406050 cites W2065462707 @default.
• W2912406050 cites W2073196482 @default.
• W2912406050 cites W2073282855 @default.
• W2912406050 cites W2073603634 @default.
• W2912406050 cites W2082239705 @default.
• W2912406050 cites W2109260915 @default.
• W2912406050 cites W2114843025 @default.
• W2912406050 cites W2117374458 @default.
• W2912406050 cites W2125323026 @default.
• W2912406050 cites W2134866235 @default.
• W2912406050 cites W2144283689 @default.
• W2912406050 cites W2149441684 @default.
• W2912406050 cites W2160545992 @default.
• W2912406050 cites W2161183734 @default.
• W2912406050 cites W2162102504 @default.
• W2912406050 cites W2174255814 @default.
• W2912406050 cites W2262414037 @default.
• W2912406050 cites W2301929217 @default.
• W2912406050 cites W2335162820 @default.
• W2912406050 cites W2354594952 @default.
• W2912406050 cites W2359508303 @default.
• W2912406050 cites W2411385452 @default.
• W2912406050 cites W2415024679 @default.
• W2912406050 cites W2491564669 @default.
• W2912406050 cites W2531201728 @default.
• W2912406050 cites W2807481509 @default.
• W2912406050 cites W4211199921 @default.
• W2912406050 doi "https://doi.org/10.18632/oncotarget.26675" @default.
• W2912406050 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6402718" @default.
• W2912406050 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30858927" @default.
• W2912406050 hasPublicationYear "2019" @default.
• W2912406050 type Work @default.
• W2912406050 sameAs 2912406050 @default.
• W2912406050 citedByCount "9" @default.
• W2912406050 countsByYear W29124060502019 @default.
• W2912406050 countsByYear W29124060502020 @default.
• W2912406050 countsByYear W29124060502021 @default.
• W2912406050 countsByYear W29124060502022 @default.
• W2912406050 countsByYear W29124060502023 @default.
• W2912406050 crossrefType "journal-article" @default.
• W2912406050 hasAuthorship W2912406050A5012988855 @default.
• W2912406050 hasAuthorship W2912406050A5020154971 @default.
• W2912406050 hasAuthorship W2912406050A5038948324 @default.
• W2912406050 hasAuthorship W2912406050A5057750122 @default.
• W2912406050 hasAuthorship W2912406050A5071809519 @default.
• W2912406050 hasAuthorship W2912406050A5079464303 @default.
• W2912406050 hasAuthorship W2912406050A5091355914 @default.
• W2912406050 hasBestOaLocation W29124060501 @default.
• W2912406050 hasConcept C104317684 @default.
• W2912406050 hasConcept C121608353 @default.
• W2912406050 hasConcept C142724271 @default.
• W2912406050 hasConcept C143998085 @default.
• W2912406050 hasConcept C150194340 @default.
• W2912406050 hasConcept C16930146 @default.
• W2912406050 hasConcept C18431079 @default.
• W2912406050 hasConcept C186836561 @default.
• W2912406050 hasConcept C193270364 @default.
• W2912406050 hasConcept C203014093 @default.
• W2912406050 hasConcept C204232928 @default.
• W2912406050 hasConcept C2779013556 @default.
• W2912406050 hasConcept C502942594 @default.
• W2912406050 hasConcept C526805850 @default.
• W2912406050 hasConcept C54355233 @default.
• W2912406050 hasConcept C71924100 @default.
• W2912406050 hasConcept C81439078 @default.
• W2912406050 hasConcept C86803240 @default.
• W2912406050 hasConcept C95371953 @default.
• W2912406050 hasConceptScore W2912406050C104317684 @default.
• W2912406050 hasConceptScore W2912406050C121608353 @default.
• W2912406050 hasConceptScore W2912406050C142724271 @default.
• W2912406050 hasConceptScore W2912406050C143998085 @default.
• W2912406050 hasConceptScore W2912406050C150194340 @default.
• W2912406050 hasConceptScore W2912406050C16930146 @default.
• W2912406050 hasConceptScore W2912406050C18431079 @default.

• next