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- W2912421035 abstract "This chapter describes the use of optogenetic heterodimerization in single cells within whole-vertebrate embryos. This method allows the use of light to reversibly bind together an “anchor” protein and a “bait” protein. Proteins can therefore be directed to specific subcellular compartments, altering biological processes such as cell polarity and signaling. I detail methods for achieving transient expression of fusion proteins encoding the phytochrome heterodimerization system in early zebrafish embryos (Buckley et al., Dev Cell 36(1):117–126, 2016) and describe the imaging parameters used to achieve subcellular light patterning." @default.
- W2912421035 created "2019-02-21" @default.
- W2912421035 creator A5044263682 @default.
- W2912421035 date "2019-01-01" @default.
- W2912421035 modified "2023-09-26" @default.
- W2912421035 title "Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos" @default.
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- W2912421035 doi "https://doi.org/10.1007/978-1-4939-9009-2_10" @default.
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