FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912446726> ?p ?o ?g. }

• W2912446726 endingPage "521" @default.
• W2912446726 startingPage "512" @default.
• W2912446726 abstract "Abstract Human fibrin hydrogels are a popular choice for use as a biomaterial within tissue engineered constructs because they are biocompatible, nonxenogenic, autologous use compatible, and biodegradable. We have recently demonstrated the ability to culture induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium on fibrin hydrogels. However, iPSCs themselves have relatively few substrate options (e.g., laminin) for expansion in adherent cell culture for use in cell therapy. To address this, we investigated the potential of culturing iPSCs on fibrin hydrogels for three-dimensional applications and further examined the use of fibrinogen, the soluble precursor protein, as a coating substrate for traditional adherent cell culture. iPSCs successfully adhered to and proliferated on fibrin hydrogels. The two-dimensional culture with fibrinogen allows for immediate adaption of culture models to a nonxenogeneic model. Similarly, multiple commercially available iPSC lines adhered to and proliferated on fibrinogen coated surfaces. iPSCs cultured on fibrinogen expressed similar levels of the pluripotent stem cell markers SSea4 (98.7% ± 1.8%), Oct3/4 (97.3% ± 3.8%), TRA1-60 (92.2% ± 5.3%), and NANOG (96.0% ± 3.9%) compared with iPSCs on Geltrex. Using a trilineage differentiation assay, we found no difference in the ability of iPSCs grown on fibrinogen or Geltrex to differentiate to endoderm, mesoderm, or ectoderm. Finally, we demonstrated the ability to differentiate iPSCs to endothelial cells using only fibrinogen coated plates. On the basis of these data, we conclude that human fibrinogen provides a readily available and inexpensive alternative to laminin-based products for the growth, expansion, and differentiation of iPSCs for use in research and clinical cell therapy applications. Stem Cells Translational Medicine 2019;8:512–521" @default.
• W2912446726 created "2019-02-21" @default.
• W2912446726 creator A5005606682 @default.
• W2912446726 creator A5017937304 @default.
• W2912446726 creator A5033657453 @default.
• W2912446726 creator A5047533635 @default.
• W2912446726 creator A5051517051 @default.
• W2912446726 creator A5054685149 @default.
• W2912446726 creator A5055125250 @default.
• W2912446726 creator A5067495927 @default.
• W2912446726 creator A5073326828 @default.
• W2912446726 creator A5082704917 @default.
• W2912446726 creator A5091114022 @default.
• W2912446726 creator A5091648076 @default.
• W2912446726 date "2019-02-15" @default.
• W2912446726 modified "2023-10-16" @default.
• W2912446726 title "Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions" @default.
• W2912446726 cites W126153012 @default.
• W2912446726 cites W1571415053 @default.
• W2912446726 cites W1596065641 @default.
• W2912446726 cites W1926498138 @default.
• W2912446726 cites W1973474133 @default.
• W2912446726 cites W1973626590 @default.
• W2912446726 cites W1984353324 @default.
• W2912446726 cites W1990330218 @default.
• W2912446726 cites W2002095196 @default.
• W2912446726 cites W2010201046 @default.
• W2912446726 cites W2015257945 @default.
• W2912446726 cites W2035895307 @default.
• W2912446726 cites W2078132464 @default.
• W2912446726 cites W2081162544 @default.
• W2912446726 cites W2082143463 @default.
• W2912446726 cites W2089299403 @default.
• W2912446726 cites W2091262660 @default.
• W2912446726 cites W2099713630 @default.
• W2912446726 cites W2111482063 @default.
• W2912446726 cites W2120121992 @default.
• W2912446726 cites W2132398504 @default.
• W2912446726 cites W2141768176 @default.
• W2912446726 cites W2144643123 @default.
• W2912446726 cites W2149042084 @default.
• W2912446726 cites W2167477836 @default.
• W2912446726 cites W2191479718 @default.
• W2912446726 cites W2214361901 @default.
• W2912446726 cites W2405682749 @default.
• W2912446726 cites W2419292400 @default.
• W2912446726 cites W2463659305 @default.
• W2912446726 cites W2525683017 @default.
• W2912446726 cites W2560216037 @default.
• W2912446726 cites W2564778420 @default.
• W2912446726 cites W2572949365 @default.
• W2912446726 cites W2622298072 @default.
• W2912446726 cites W2737132772 @default.
• W2912446726 cites W2773993757 @default.
• W2912446726 cites W2799801940 @default.
• W2912446726 cites W2943659207 @default.
• W2912446726 doi "https://doi.org/10.1002/sctm.18-0189" @default.
• W2912446726 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6525556" @default.
• W2912446726 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30768863" @default.
• W2912446726 hasPublicationYear "2019" @default.
• W2912446726 type Work @default.
• W2912446726 sameAs 2912446726 @default.
• W2912446726 citedByCount "12" @default.
• W2912446726 countsByYear W29124467262020 @default.
• W2912446726 countsByYear W29124467262021 @default.
• W2912446726 countsByYear W29124467262022 @default.
• W2912446726 countsByYear W29124467262023 @default.
• W2912446726 crossrefType "journal-article" @default.
• W2912446726 hasAuthorship W2912446726A5005606682 @default.
• W2912446726 hasAuthorship W2912446726A5017937304 @default.
• W2912446726 hasAuthorship W2912446726A5033657453 @default.
• W2912446726 hasAuthorship W2912446726A5047533635 @default.
• W2912446726 hasAuthorship W2912446726A5051517051 @default.
• W2912446726 hasAuthorship W2912446726A5054685149 @default.
• W2912446726 hasAuthorship W2912446726A5055125250 @default.
• W2912446726 hasAuthorship W2912446726A5067495927 @default.
• W2912446726 hasAuthorship W2912446726A5073326828 @default.
• W2912446726 hasAuthorship W2912446726A5082704917 @default.
• W2912446726 hasAuthorship W2912446726A5091114022 @default.
• W2912446726 hasAuthorship W2912446726A5091648076 @default.
• W2912446726 hasBestOaLocation W29124467261 @default.
• W2912446726 hasConcept C104317684 @default.
• W2912446726 hasConcept C107459253 @default.
• W2912446726 hasConcept C108586683 @default.
• W2912446726 hasConcept C145103041 @default.
• W2912446726 hasConcept C148738053 @default.
• W2912446726 hasConcept C178790620 @default.
• W2912446726 hasConcept C185592680 @default.
• W2912446726 hasConcept C189165786 @default.
• W2912446726 hasConcept C203014093 @default.
• W2912446726 hasConcept C2779036427 @default.
• W2912446726 hasConcept C2779814568 @default.
• W2912446726 hasConcept C28328180 @default.
• W2912446726 hasConcept C54173615 @default.
• W2912446726 hasConcept C55493867 @default.
• W2912446726 hasConcept C86803240 @default.
• W2912446726 hasConcept C95444343 @default.
• W2912446726 hasConceptScore W2912446726C104317684 @default.

• next